间充质干细胞与巨噬细胞的共培养技术  

Co-culture technology of mesenchymal stem cells and macrophages

作　　者：杨小倩 宋爱梅 宋晖 Yang Xiaoqian;Song Aimei;Song Hui(School of Stomatology,Cheeloo College of Medicine,Shandong University,Jinan 250012,Shandong Province,China;Shandong Provincial Key Laboratory of Oral Tissue Regeneration,Jinan 250012,Shandong Province,China;Shandong Provincial Laboratory of Oral Biomaterials and Tissue Regeneration Engineering,Jinan 250012,Shandong Province,China;Shandong Provincial Clinical Medical Research Center for Oral Diseases,Jinan 250012,Shandong Province,China)

机构地区：[1]山东大学齐鲁医学院口腔医学院,山东省济南市250012 [2]山东省口腔组织再生重点实验室,山东省济南市250012 [3]山东省口腔生物材料与组织再生工程实验室,山东省济南市250012 [4]山东省口腔疾病临床医学研究中心,山东省济南市250012

出　　处：《中国组织工程研究》2024年第31期5055-5062,共8页Chinese Journal of Tissue Engineering Research

基　　金：山东省自然科学基金(ZR2020MH184),项目负责人:宋爱梅。

摘　　要：背景:间充质干细胞与巨噬细胞共培养环境中,间充质干细胞能够促进巨噬细胞向抗炎型巨噬细胞极化减轻炎症反应,巨噬细胞能够促进间充质干细胞成骨分化,两者共培养在调节免疫系统和促进组织再生中发挥重要的作用。目的:综述间充质干细胞与巨噬细胞共培养方法、影响因素及相互调控的可能机制,为间充质干细胞和巨噬细胞共培养在组织工程中的应用提供理论依据以及实验方法参考。方法:第一作者在2023年1-9月应用计算机在PubMed和中国知网数据库中检索1970年1月至2023年9月相关文献,以“mesenchymal stem cells,macrophages,co-culture”为英文检索词,以“间充质干细胞,巨噬细胞,共培养”为中文检索词,最终纳入63篇文献进行分析。结果与结论:①体外间充质干细胞与巨噬细胞共培养体系根据模型可分为直接接触和间接接触共培养,根据维度可分为二维和三维细胞共培养。②间充质干细胞与巨噬细胞共培养能够促进巨噬细胞向M2型极化,增强间充质干细胞的成骨作用。③在共培养模型中,共培养方法、共培养比例与时间、巨噬细胞的表型及细胞来源和条件的不同均影响巨噬细胞的免疫调节和间充质干细胞的成骨作用。④共培养中细胞相互作用可能通过细胞分泌的可溶性因子、细胞外囊泡、细胞-细胞接触和代谢途径调节巨噬细胞的免疫功能,对间充质干细胞的增殖、迁移和成骨等生物学功能产生一定影响。⑤间充质干细胞和巨噬细胞能改善急性心肌梗死后的心功能、促进上皮创面愈合、减轻肺部炎症、改善肾功能和促进骨修复。⑥间充质干细胞和巨噬细胞共培养仍存在一些问题:例如共培养的条件选择、共培养细胞良好细胞状态的保持和相互作用等。⑦间充质干细胞与巨噬细胞共培养改善局部炎症微环境,促进组织再生修复,在组织工程中具有广阔的应�BACKGROUND:In the co-culture environment of mesenchymal stem cells and macrophages,mesenchymal stem cells can promote the polarization of macrophages into anti-inflammatory macrophages to reduce inflammation,and macrophages can promote the osteogenic differentiation of mesenchymal stem cells.The co-culture of both plays an important role in regulating the immune system and promoting tissue regeneration.OBJECTIVE:To summarize the methods,influencing factors and possible mechanisms of co-culture between mesenchymal stem cells and macrophages,and to provide theoretical basis and experimental methods for the application of co-culture of mesenchymal stem cells and macrophages in tissue engineering.METHODS:The first author searched the relevant articles published from January 1970 to September 2023 in PubMed and CNK by computer from January to September 2023.The Chinese and English key words were“mesenchymal stem cells,macrophages,co-culture”.Finally,63 articles were included and analyzed.RESULTS AND CONCLUSION:(1)In vitro co-culture of mesenchymal stem cells and macrophages can be divided into direct contact co-culture and indirect contact co-culture according to the model,and two-dimensional cell co-culture and three-dimensional cell co-culture according to the dimension.(2)The co-culture of mesenchymal stem cells and macrophages can promote the polarization of macrophages towards M2 type and enhance the osteogenic effect of mesenchymal stem cells.(3)In the co-culture model,the methods of co-culture,the proportion and time of co-culture,the phenotype of macrophages,and the cell source and conditions all affected the immune regulation of macrophages and the osteogenesis of mesenchymal stem cells.(4)Cell interaction in co-culture may regulate the immune function of macrophages,proliferation,migration and osteogenesis of mesenchymal stem cells through cell-secreted soluble factors,extracellular vesicles,cell-cell contact,and metabolic pathways.(5)Mesenchymal stem cells and macrophages can enhance cardiac function af

关 键 词：间充质干细胞 巨噬细胞 细胞共培养 直接接触 旁分泌 三维共培养体系 组织再生 组织工程 

分 类 号：R459.9[医药卫生—治疗学] R363[医药卫生—临床医学] R364