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作 者:王婧尊 黄志 曹世坤 郑菲 徐红伟[1] 郭鹏辉[1] 杨具田[1] 蔡勇[1,2] WANG Jingzun;HUANG Zhi;CAO Shikun;ZHENG Fei;XU Hongwei;GUO Penghui;YANG Jutian;CAI Yong(School of Life Sciences and Engineering,Northwest Minzu University,Lanzhou,Gansu 730030,China;Department of Experimental Teaching Northwest Minzu University,Lanzhou,Gansu 730030,China)
机构地区:[1]西北民族大学生命科学与工程学院,甘肃兰州730030 [2]西北民族大学实验教学部,甘肃兰州730030
出 处:《畜牧兽医杂志》2024年第6期1-6,共6页Journal of Animal Science and Veterinary Medicine
基 金:甘肃省高校产业支撑计划项目(2023CYZC-08);中央高校基本科研业务费专项(31920220070、31920230166、31920220057);国家民委中青年英才培养计划项目(民人事字〔2022〕366);大学生创新创业训练计划项目(X202410742292、X202410742298)。
摘 要:肌肉生长抑制素(Myostatin,MSTN)是肌肉生长的负调节因子,抑制了成肌细胞的增殖和分化。本文研究MSTN可选作兰州大尾羊生长性状候选基因的可能性,PCR扩增及PCR-SSCP技术检测536只兰州大尾羊MSTN基因多态性,并分析其群体分布规律。结果:(1)兰州大尾羊MSTN基因中检测到2个SNP位点,其中g.119286026C>A的等位基因型为CC和CA,突变位点CC基因型胸宽和胸围显著高于CA基因型(P<0.05);g.119286166A>G的等位基因型为AA和AG,突变位点AG基因型体斜长和体重显著高于AA基因型(P<0.05),两位点均呈现低多态性。(2)其中2个基因型组合(A1和C1)对兰州大尾羊生长性状具有显著影响,C1组基因型组合体斜长、胸围、胸宽和胸深均显著高于B1组基因型组合(P<0.05),在各基因型之间其他生长性状差异均不显著(P>0.05)。结果表明,MSTN基因多态性与兰州大尾羊的体斜长、体重、胸围和胸宽显著相关,可作为其生长性状的有效遗传标记位点。Myostatin(MSTN)is a negative regulator of muscle growth that inhibits myoblast proliferation and differentiation.In order to explore the possibility of MSTN gene as a candidate for growth traits in Lanzhou fat-tailed sheep,PCR amplification and PCR-SSCP technology were used to detect the polymorphisms of MSTN gene in 536 Lanzhou fat-tailed sheep,and their population distribution was analyzed.Results:(1)Two SNPs were detected in the MSTN gene of Lanzhou fat-tailed sheep,among which the alleles of g.119286026C>A were CC and CA,and the CC genotype’s chest width and circumference were prominently higher than CA genotype(P<0.05),the alleles of g.119286166A>G locus were AA and AG,and the AG genotype’s body oblique length and weight of the were prominently higher than AA genotype(P<0.05),both points showed low polymorphism.(2)Genotypic combination analysis showed that two genotype combinations(A1 and C1)had a significant effect on the growth traits of Lanzhou fat-tailed sheep,and the body oblique length and chest circumference,width and depth of the genotype group C1 were prominently higher than genotype group B1(P<0.05),and there were noprominent differences in other growth traits between genotype groups(P>0.05).The results showed that MSTN gene polymor-phisms were prominently related with body oblique length,body weight,chest circumference and width of Lanzhou fat-tailed sheep,which could be used as an effective genetic marker site for their growth traits.
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