桑黄通泻方对糖尿病伴抑郁状态大鼠海马线粒体自噬的影响  

Effects of Sanghuang Tongxie Prescription on mitophagy in hippocampus of diabetic depression rats with depression

作　　者：蒲菁菁 常宏 王萌 李继安 Pu Jingjing;Chang Hong;Wang Meng;Li Ji'an(College of Traditional Chinese Medicine of North China University of Science and Technology,Tangshan 063210,China;Hebei Key Laboratory of Integrated Traditional Chinese and Western Medicine for Diabetes and Its Complications,Tangshan 063210,China)

机构地区：[1]华北理工大学中医学院,唐山063210 [2]河北省中西医结合防治糖尿病及其并发症重点实验室,唐山063210

出　　处：《国际中医中药杂志》2024年第11期1439-1443,共5页International Journal of Traditional Chinese Medicine

基　　金：河北省自然科学基金(H2023209058);科技部对发展中国家常规性科技援助项目(KY201904005)。

摘　　要：目的观察桑黄通泻方对糖尿病伴抑郁状态大鼠海马线粒体自噬的影响。方法将32只大鼠按随机数字表法分为空白组、模型组、二甲双胍组和桑黄通泻方组,每组8只。除空白组外,其余各组采用链脲佐菌素、高脂饲料、慢性轻度不可预知应激建立糖尿病伴抑郁状态大鼠模型。造模成功后,二甲双胍组灌胃二甲双胍溶液100 mg/kg,桑黄通泻方组灌胃桑黄通泻方混悬液5.7 g/kg,空白组和模型组大鼠灌胃等体积的生理盐水,1次/d,连续干预12周。给药结束后,检测空腹血糖(FPG)、2 hPG和蔗糖偏好率;Western blot法检测海马区线粒体自噬相关蛋白PTEN诱导激酶(PINK-1)、E3泛素连接酶(Parkin)、螯合体1(P62)、自噬和beclin1调节器(Beclin-1)、微管相关蛋白1轻链3 beta(LC32/1)蛋白表达。结果与模型组比较,桑黄通泻方组大鼠FPG、2 hPG水平降低(P<0.01),蔗糖偏好率升高(P<0.01);海马区PINK-1、Parkin、P62、Beclin-1、LC32/1蛋白表达降低(P<0.01)。结论糖尿病大鼠伴随不同程度的快感缺失和抑郁状态,桑黄通泻方可改善糖尿病大鼠的抑郁状态,其作用机制可能与下调海马线粒体自噬水平、改善线粒体功能有关。Objective To observe the effect of Sanghuang Tongxie Prescription on mitophagy in hippocampus of diabetic depression rats.Methods Totally 32 SD rats were divided into control group,model group,metformin group and Sanghuang Tongxie Prescription group according to random number table method,with 8 rats in each group.The model of diabetic depression rats with depression was established by streptozotocin(STZ),high-fat diet and chronic unpredictable mild stress except control group.Metformin group was given metformin 100 mg/kg,Sanghuang Tongxie Prescription group was given Sanghuang Tongxie Prescription suspension 5.7 g/kg,control group and model group were given the same volume of normal saline by gavage,once a day for 12 weeks.After administration,fasting plasma glucose(FPG),2-hour postprandial blood glucose(2 hPG)and sucrose preference rate were measured after treatment;the expressions of PINK-1,Parkin,P62,Beclin-1 and LC32/1 were detected by Western blot.Results Compared with the model group,the expressions of FPG and PG2h in Sanghuang Tongxie Prescription group decreased(P<0.01),and the sucrose preference rate increased(P<0.01);the expressions of PINK-1,Parkin,P62,Beclin-1 and LC32/1 in hippocampus decreased(P<0.01).Conclusions Diabetic rats are accompanied by varying degrees of pleasure loss and depressive state.Sanghuang Tongxie Prescription can improve the depressive state of rats.Its mechanism may be related to down-regulating the level of mitochondrial autophagy in the hippocampus and improving mitochondrial function.

关 键 词：糖尿病 抑郁症 桑黄通泻方 海马 线粒体自噬 大鼠 

分 类 号：R285.5[医药卫生—中药学]