丹蛭降糖胶囊对db/db小鼠胰腺组织线粒体氧化损伤的影响  

Effects of Danzhi Jiangtang Capsules on mitochondrial oxidation damage in db/db mice

作　　者：吕溪涓 许奇 阮诺冰 李金菊 毕正 李瑜璠 方朝晖[2,3] Lyu Xijuan;Xu Qi;Ruan Nuobing;Li Jinju;Bi Zheng;Li Yufan;Fang Zhaohui(First Clinical Medical College,Anhui University of Chinese Medicine,Hefei 230031,China;Department of Endocrinology,First Affiliated Hospital of Anhui University of Chinese Medicine,Hefei 230031,China;Key Laboratory of Xin'an Medical Education(Anhui University of Chinese Medicine),Hefei 230038,China)

机构地区：[1]安徽中医药大学第一临床医学院,合肥230031 [2]安徽中医药大学第一附属医院内分泌科,合肥230031 [3]新安医学教育部重点实验室(安徽中医药大学),合肥230038

出　　处：《国际中医中药杂志》2024年第11期1444-1449,共6页International Journal of Traditional Chinese Medicine

基　　金：国家自然科学基金(81774286);安徽省高校协同创新项目(GXXT-2020-025)。

摘　　要：目的探讨丹蛭降糖胶囊对db/db糖尿病小鼠糖代谢及线粒体氧化应激水平的调节作用。方法将32只db/db小鼠按随机数字表法分为模型组、阳性对照组及丹蛭降糖胶囊低、中、高剂量组,同时选择8只同性同周龄C57BL/6小鼠作为空白组。二甲双胍组灌胃二甲双胍溶液0.1 g/kg,丹蛭降糖胶囊高、中、低剂量组分别灌胃0.99、0.49、0.25 g/kg丹蛭降糖胶囊溶液,模型组和空白组灌胃等体积的生理盐水,1次/d,连续灌胃12周。检测各组小鼠体重和空腹血糖(FBG)变化;ELISA法检测空腹胰岛素(FINS)水平,计算胰岛素抵抗指数(HOMA-IR);使用试剂盒检测各组小鼠胰腺组织SOD、MDA水平;采用HE染色观察胰腺组织病理学形态,透射电镜观察胰腺线粒体超微结构;Western blot法检测胰腺组织中p-AMPK、AMPK蛋白表达。结果与模型组比较,给药干预8、12周后,丹蛭降糖胶囊高剂量组小鼠体重降低(P<0.05或P<0.01),FBG水平降低(P<0.01);丹蛭降糖胶囊高、中剂量组小鼠FINS、HOMA-IR降低(P<0.01),丹蛭降糖胶囊高、中、低剂量组小鼠SOD活性升高、MDA水平降低(P<0.01);丹蛭降糖胶囊高、中剂量组p-AMPK、AMPK表达升高(P<0.01),丹蛭降糖胶囊低剂量组AMPK表达升高(P<0.01)。结论丹蛭降糖胶囊可提高db/db小鼠胰腺组织中SOD活性,降低MDA含量,激活AMPK信号通路,提示丹蛭降糖胶囊通过抑制氧化应激水平和改善线粒体功能,改善胰岛素抵抗,恢复葡萄糖稳态。Objective To explore the regulatory effects of Danzhi Jiangtang Capsules on glucose metabolism and mitochondrial oxidative stress levels in db/db mice.Methods Totally 32 db/db mice were randomly divided into model group,positive control group,and Danzhi Jiangtang Capsule low-,medium-and high-dosage groups;at the same time,8 same-sex C57BL/6 mice of the same week age were selected as the blank group.The metformin group was filled with metformin solution 0.1 g/kg,and the Danzhi Jiangtang Capsule low-,medium-and high-dosage groups were injected with 0.99,0.49 and 0.25 g/kg Danzhi Jiangtang Capsule solution respectively.The model group and the blank group received the same volume of physiological saline solution for the gavage,1 time/d,continuous 12 weeks.The weight and fasting blood sugar(FBG)changes in each group of mice were detected;the serum insulin(FINS)level was detected by ELISA method and the insulin resistance index(HOMA-IR)was calculated;kits were used to detect the pancreatic tissue SOD and MDA levels of each group of mice;HE staining was used to observe pathological morphology of pancreatic tissue.Transmission electron microscopy was used to observe the ultrastructure of pancreatic mitochondria;Western blot method was used to detect the expressions of p-AMPK and AMPK proteins in pancreatic tissue.Results Compared with the model group,after 8 or 12 weeks of drug administration,the weight of mice in the Danzhi Jiangtang Capsule high-dosage group decreased(P<0.05 or P<0.01),and the level of FBG decreased(P<0.01);FINS and HOMA-IR in the Danzhi Jiangtang Capsule high-and medium-dosage groups decreased(P<0.01),the SOD activity in Danzhi Jiangtang Capsule high-medium-and low-dosage groups increased,and the level of MDA decreased(P<0.01);the expressions of p-AMPK and AMPK in Danzhi Jiangtang Capsule high-and medium-dosage groups increased(P<0.01),the expression of AMPK in Danzhi Jiangtang Capsule low-dosage group increased(P<0.01).Conclusion Danzhi Jiangtang Capsule can improve SOD activity in pancreatic tissue

关 键 词：糖尿病 2型 丹蛭降糖胶囊 线粒体 氧化性应激 胰岛素抵抗 糖代谢 DB/DB小鼠 

分 类 号：R285.5[医药卫生—中药学]