蒙医温针对抑郁模型大鼠细胞焦亡及炎症的影响  

Effects of Mongolian medicine warm acupuncture on pyroptosis and inflammation in depression model rats

作　　者：张丽霞 于久旺 张婷婷 白永峰 Zhang Lixia;Yu Jiuwang;Zhang Tingting;Bai Yongfeng(Mongolian Medicine Chronic Disease Diagnosis and Treatment Center,Hohhot Mongolian Medicine of Traditional Chinese Medicine Hospital,Hohhot 010010,China;Scientific Research Department,Hohhot Mongolian Medicine of Traditional Chinese Medicine Hospital,Hohhot 010010,China;Pathology Department,Xilinguole Mongolian Hospital,Xilinhot 026000,China)

机构地区：[1]呼和浩特市蒙医中医医院蒙医慢病诊疗中心,呼和浩特010010 [2]呼和浩特市蒙医中医医院科研部,呼和浩特010010 [3]锡林郭勒盟蒙医医院病理科,锡林浩特026000

出　　处：《国际中医中药杂志》2024年第11期1469-1474,共6页International Journal of Traditional Chinese Medicine

摘　　要：目的观察蒙医温针对抑郁模型大鼠行为学及海马体中核苷酸结合寡聚结构域样受体蛋白3(NLRP3)、凋亡相关斑点样蛋白(ASC)、Caspase-1、焦亡效应蛋白消皮素D(GSDMD)及炎症因子的影响,探讨其作用机制。方法将40只SD雄性大鼠按随机数字表法分为对照组、模型组、温针组和氟西汀组,每组10只。除对照组外,其余各组建立慢性不可预测性应激模型。每天应激刺激1 h后,温针组进行蒙医温针干预,氟西汀组灌胃盐酸氟西汀2.1 mg/kg,对照组和模型组灌胃等体积蒸馏水,1次/d,持续21 d。测定大鼠体重,并进行糖水消耗实验、旷场实验及Morris水迷宫实验评定其行为学,采用ELISA法测定血清IL-1β、IL-18、IL-6、TNF-α水平,采用Western blot法和PCR法检测海马NLRP3、ASC、Caspase-1、GSDMD、IL-1蛋白及mRNA水平。结果与模型组比较,温针组、氟西汀组大鼠体重增加(P<0.05),糖水消耗量增加(P<0.05),垂直运动次数、水平穿越格数增加(P<0.05),逃避潜伏期缩短(P<0.05),穿越平台次数增多(P<0.05);血清IL-1β、IL-18、TNF-α、IL-6水平降低(P<0.05);大鼠海马区焦亡蛋白NLRP3、Caspase-1、ASC、GSDMD、IL-1β蛋白及mRNA表达降低(P<0.05)。结论蒙医温针可通过减少NLRP3介导的焦亡和炎症预防大鼠抑郁样行为。Objective To observe the effects of Mongolian medicine warm acupuncture treatment on the behavior and NLRP3,ASC,Caspase-1,GSDMD,and inflammatory factors in hippocampus of the depression model rats;To explore the mechanism.Methods Totally 40 male SD rats were divided into control group,model group,warm acupuncture group,and fluoxetine group using a random number table method,with 10 rats in each group.Except for the control group,chronic unpredictable stress models were established in all other groups.After 1 hour of daily stress stimulation,the warm acupuncture group received Mongolian medicine warm acupuncture intervention.The fluoxetine group was orally administered with 2.1 mg/kg fluoxetine hydrochloride,while the control group and model group were orally administered with equal volumes of distilled water once a day for 21 days.The body weight of rats was measured,and their behavior was evaluated by sugar water consumption test,open field test,and Morris water maze test.ELISA was used to measure the levels of IL-1β,IL-18,IL-6,and TNF-αin the serum.Western blot and PCR were used to detect the protein and mRNA levels of NLRP3,ASC,Caspase-1,GSDMD,and IL-1 in the hippocampus.Results Compared with the model group,the warm acupuncture group and fluoxetine group showed an increase in body weight(P<0.05),sugar water consumption(P<0.05),vertical movement frequency and horizontal crossing grid number(P<0.05),shortened escape latency(P<0.05),and increased crossing platform frequency(P<0.05);the levels of serum IL-1β,IL-18,TNF-α,and IL-6 decreased(P<0.05);the protein and mRNA expressions of NLRP3,Caspase-1,ASC,GSDMD,IL-1βin the hippocampus of rats decreased(P<0.05).Conclusion Mongolian medicine warm acupuncture can prevent depressive behaviors in rats by reducing NLRP3-mediated cell death and inflammation.

关 键 词：温针疗法 抑郁症 细胞焦亡 炎症 大鼠 

分 类 号：R29[医药卫生—民族医学]