基于HPLC指纹图谱和多元统计学分析的马兜铃蜜炙前后化学成分变化研究  

Study on the changes of chemical components of Aristolochia contorta before and after honey processing based on HPLC fingerprint and multivariate statistical analysis

作　　者：刘晓霞 冯涌微 位翠杰 吴春秀 何民友 潘礼业 孙冬梅 李振雨 Liu Xiaoxia;Feng Yongwei;Wei Cuijie;Wu Chunxiu;He Minyou;Pan Liye;Sun Dongmei;Li Zhenyu(Guangdong Yifang Pharmaceutical Co.,Ltd.,Guangdong Provincial Key Laboratory of Traditional Chinese Medicine Formula,Foshan 528244,China;Longxi Yifang Pharmaceutical Co.,Ltd.,Dingxi 748100,China)

机构地区：[1]广东一方制药有限公司,广东省中药配方颗粒企业重点实验室,佛山528244 [2]陇西一方制药有限公司,定西748100

出　　处：《国际中医中药杂志》2024年第11期1485-1492,共8页International Journal of Traditional Chinese Medicine

基　　金：2022年佛山市南海区重点领域科技攻关专项(南科〔2023〕20号-18);2023年佛山市自筹经费类科技创新项目(2320001007554)。

摘　　要：目的建立马兜铃和蜜马兜铃HPLC指纹图谱,并结合多元统计学方法分析马兜铃蜜炙前后的化学成分变化,为马兜铃炮制减毒研究提供参考。方法采用HPLC法建立11批马兜铃和蜜马兜铃HPLC指纹图谱,经聚类分析、主成分分析、正交偏最小二乘法-判别式分析(OPLS-DA)及统计学方法,分析马兜铃蜜炙前后化学成分变化。结果马兜铃和蜜马兜铃指纹图谱均标定14个共有峰,其中指认7个成分。聚类分析、主成分分析均可明显区马兜铃和蜜马兜铃样品,OPLS-DA确定并筛选出7个差异性标志物,差异显著性排序分别为峰3>峰7(7-羟基马兜铃酸A)>峰5(马兜铃酸C)>峰8(马兜铃酸D)>峰6>峰2(木兰花碱)>峰14(马兜铃酸I)。蜜炙后马兜铃指纹图谱峰2、3、5、6、7、8、14所代表的化学成分含量降低(P<0.05)。结论该方法操作方便简单,专属性强,能用于马兜铃和蜜马兜铃的指纹图谱分析,且能有效区分马兜铃和蜜马兜铃,并为马兜铃蜜炙减毒的炮制研究提供参考。Objective To establish HPLC fingerprints of Aristolochia contorta and honey-processed Aristolochia contorta;To analyze the changes of chemical components before and after honey processing with multivariate statistics;To provide a reference for the study on the toxicity reduction of Aristolochia contorta.Methods The fingerprints of 11 batches of Aristolochia contorta and honey-processed Aristolochia contorta were established through HPLC.Clustering analysis(HCA),principal component analysis(PCA),orthogonal partial least squares discriminant analysis(OPLS-DA)and independent sample t-test were used to compare the changes of chemical components of Aristolochia contorta before and after honey processing.Results The results showed that there were 14 common peaks in the fingerprints of Aristolochia contorta and Aristolochia contorta.7 common peaks were identified.Both HCA and PCA could clearly distinguish the samples of Aristolochia contorta before and after honey processing.OPLS-DA found and screened 7 differential markers,and the order of difference significance was peak 3>peak 7(7-hydroxy aristolochic acid A)>peak 5(aristolochic acid C)>peak 8(aristolochic acid D)>peak 6>peak 2(Magnolia alkaloid)>peak 14(aristolochic acidⅠ).After honey processing,the content of chemical components represented by peaks 2,3,5,6,7,8 and 14 decreased(P<0.05).Conclusion This method is simple and specific,which can be used for the fingerprint analysis of Aristolochia contorta and honey-processed Aristolochia contorta,and can effectively distinguish Aristolochia contorta and honey-processed Aristolochia contorta,and provide a reference for the processing research of toxicity reduction of Aristolochia contorta honey processing.

关 键 词：炮制(中药) 马兜铃 蜜炙 HPLC指纹图谱 相似度 正交偏最小二乘法-判别分析 

分 类 号：R283.1[医药卫生—中药学]