不同检测周期对HBsAg ELISA检测结果的影响  

The influence of different detection cycles on the detection results of HBsAg ELISA

作　　者：邓燕清[1] 万芷婷 黄伯泉[1] 梁浩坚[1] 杜荣松[1] 李仲平[1] 郑剑婷 许茹[1] 王敏[1] 王淏[1] DENG Yanqing;WAN Zhiting;HUANG Boquan;LIANG Haojian;DU Rongsong;LI Zhongping;ZHENG Jianting;XU Ru;WANG Min;WANG Hao(Guangzhou Blood Center,The Key Medical Laboratory of Guangzhou,Guangzhou 510095,China)

机构地区：[1]广州血液中心广州市血液安全重点实验室,广东广州510095

出　　处：《中国输血杂志》2024年第11期1301-1306,共6页Chinese Journal of Blood Transfusion

基　　金：广州市科技计划基金项目(2023A03J0553,2024A03J0150)。

摘　　要：目的考察献血者HBsAg ELISA阳性标本采样后检测周期长短对检测结果的影响。方法选取广州血液中心2023年8月10日—12月9日的献血者血液标本127044份,采用2种ELISA试剂进行HBsAg检测,通过对HBsAg双试剂阳性且S/CO值均<10的标本进行连续7 d检测,观察其S/CO值的变化。结果共检测出HBsAg ELISA双试剂阳性标本505份,其中S/CO值<10的有52份,对其连续7 d不间断检测,万泰(中位数5降低到3)和新创(中位数5降低到3)S/CO值总体呈下降趋势,HBsAg ELISA漏检率呈上升趋势(从d10上升至d7万分之1),共有13份标本在7 d检测周期内ELISA双试剂检测结果变成阴性。结论随着检测周期的延长,HBsAg ELISA检测的S/CO值呈下降趋势,HBsAg ELISA漏检率呈上升趋势。用于HBsAg ELISA检测的标本,应于采样后及时进行检测,提高血液检测质量。Objective To analyze the influence of the cycle length of hepatitis B surface antigen(HBsAg)double reagent positive samples collected from voluntary blood donors in Guangzhou on the detection results.Methods A total of 127044 blood samples from voluntary blood donors at Guangzhou Blood Center from August 10 to December 9,2023 were selected.Two ELISA reagents were used for HBsAg detection,and samples with HBsAg double reagent positive and S/CO values<10 were tested continuously for 7 days to observe the changes in their S/CO values.Results A total of 505 HBsAg double reagent positive samples were detected,of which 52 had S/CO values less than 10.After 7 consecutive days of uninterrupted testing,the S/CO values of Wantai(median 5 decreased to 3)and Xinchuang(median 5 decreased to 3)showed an overall downward trend,and the HBsAg missed detection rate showed an upward trend(from 0 on the first day to 1/10000 on the seventh day).A total of 13 cases had negative double reagent test results within the 7-day testing cycle.Conclusion With the extension of the detection cycle,the S/CO value of HBsAg detection shows a downward trend,and the missed detection rate of HBsAg shows an upward trend.Samples used for HBsAg detection should be tested promptly after sampling to improve the quality of blood testing.

关 键 词：HBSAG 检测时间 漏检率 

分 类 号：R457.1[医药卫生—治疗学] R446[医药卫生—临床医学]