Prussian Blue Nanoparticles with High Oxidase-like Activity for Simultaneous Detection of Ascorbic Acid and Alkaline Phosphatase in Real Samples  

作　　者：TANG Zeng LAI Yue LU Hao XIA Yi JIANG Xue XIONG Xiaoli XIAO Ting 

机构地区：[1]College of Chemistry and Material Science,Key Laboratory of the Evaluation and Monitoring of Southwest Land Resources,Ministry of Education,Sichuan Normal University,Chengdu 610068,P.R.China

出　　处：《Chemical Research in Chinese Universities》2024年第6期1023-1032,共10页高等学校化学研究（英文版）

基　　金：supported by the National Natural Science Foundation of China (No.22104103);the Program of Sichuan Department of Science and Technology,China (No.2022ZYFG0312);the Foundation of Sichuan Normal University,China (No.XJ20210223).

摘　　要：Prussian blue nanoparticles (PB NPs) have exhibited favorable anti-inflammatory effects in animal models,and have been proved to mimic three antioxidant enzymes: peroxidase,catalase,and superoxide dismutase.Whereas the oxidase-like activity of PB NPs has not been demonstrated.In this work,PB NPs with outstanding oxidase-like activity were demonstrated by virtue of the colorimetric substrate o-phenylenediamine (OPD).Interestingly,based on the oxidase-like activity,PB NPs can also catalytically oxidize vitamin B1 (VB1) to strongly fluorescent product in the physiological environment without the addition of other oxidants.Considering that VB1 has no toxicity and carcinogenicity comparing with OPD,a novel PB NPs-VB1 fluorescent sensor was further developed for simple,nontoxic,and rapid detection of ascorbic acid (AA) in foods and alkaline phosphatase (ALP) in human serum samples by taking advantage of the reduction of PB NPs to Prussian White by AA or enzymatic hydrolysate of ALP and ascorbic acid 2-phosphate (AA2P);leading to the decrease of fluorescence intensity.The extremely low detection limits of 44 nmol/L for AA and 72 mU/L for ALP as well as the excellent applicability in real samples were both obtained.Therefore,we envision that such a novel fluorescent probe would be potentially applied in the food analysis and clinic diagnosis fields.

关 键 词：Prussian blue nanoparticles Oxidase-like activity Fluorescence probe Ascorbic acid Alkaline phosphatase 

分 类 号：O62[理学—有机化学]