Construction of Photo- and Thermo-Responsive Polymer-MOF@Enzyme Composites for Enhancing Its Biocatalytic Performance  

作　　者：TAJWAR Muhammad Ali ALI Nasir ZHANG Xiangru JABEEN Rubina LIU Yutong SHANGGUAN Dihua QI Li 

机构地区：[1]Beijing National Laboratory for Molecular Sciences,Key Laboratory of Analytical Chemistry for Living Bio-systems,Institute of Chemistry,Chinese Academy of Sciences,Beijing 100190,P.R.China [2]School of Chemical Sciences,University of Chinese Academy of Sciences,Beijing 100049,P.R.China [3]School of Pharmacy,Xinxiang Medical University,Xinxiang 453003,P.R.China

出　　处：《Chemical Research in Chinese Universities》2024年第6期1290-1297,共8页高等学校化学研究（英文版）

基　　金：supported by the National Natural Science Foundation of China (No.22274159)。

摘　　要：Recently,metal-organic framework (MOF)@enzyme composites have attracted increasing research interest.However,the fabrication of polymers-modified MOF@enzyme composites with high bio-catalytic performance remains challenging.Herein,a stimulus-responsive polymer,poly(acrylate-3',3'-dimethyl-6-nitro-spiro-2H-1-benzopyran-2,2' indoline-1'-ester-co-N-isopropyl acrylamide) (PSPN);was in-situ polymerized in UiO-66-NH2 (UN).The dual-responsive PSPN-UN@L-ASNase composites were constructed following L-asparaginase (L-ASNase) covalently attached to the UN surface.Interestingly,under 365 nm UV irradiation at 45 ℃,the PSP moiety in PSPN underwent a trans-to-cis conformational change and the PN moiety in PSPN transferred from a stretched- to a coiled-state,generating a confinement effect that significantly enhanced the bio-catalytic performance of the composites.Compared with free L-ASNase,the composites showed a 42.0-folds increase in maximum catalytic reaction velocity.Furthermore,the PSPN-UN@L-ASNase composites demonstrated high toxicity for Jurkat leukaemia cells.The stimulus-responsive polymer-MOF@enzyme composites provide a novel avenue for controlled bio-catalysis with great potential for targeted leukaemia therapy.

关 键 词：Photo-/thermo-responsive polymer Metal-organic framework(MOF) L-Asparaginase(L-ASNase)immobilization Confinement effect Catalytic efficiency Leukaemia cell 

分 类 号：TB33[一般工业技术—材料科学与工程]