Electrospun polyvinyl alcohol fibres incorporating an antimicrobial gel for enzymatically controlled reactive oxygen species release  

作　　者：Joel Yupanqui Mieles Cian Vyas Evangelos Daskalakis Mohamed Hassan James Birkett Abdalla M.Omar Gavin Humphreys Carl Diver Paulo Bartolo 

机构地区：[1]Department of Mechanical,Aerospace and Civil Engineering,University of Manchester,Manchester M139PL,UK [2]Singapore Centre for 3D Printing,School of Mechanical and Aerospace Engineering,Nanyang Technological University,Singapore 639798,Singapore [3]Department of Chemistry,University of Manchester,Manchester M139PL,UK [4]Division of Pharmacy and Optometry,University of Manchester,Manchester M139PL,UK [5]Department of Engineering,Manchester Metropolitan University,Manchester M139PL,UK

出　　处：《Bio-Design and Manufacturing》2024年第6期899-925,共27页生物设计与制造（英文）

基　　金：supported by Matoke Holdings,the United Kingdom(UK)Engineering and Physical Sciences Research Council(EPSRC)Doctoral Prize Fellowship(No.EP/R513131/1);the Henry Royce Institute for Advanced Materials,funded through EPSRC grants(Nos.EP/R00661X/1,EP/S019367/1,EP/P025021/1,and EP/P025498/1);Ruth Edge and Kevin Warren(Dalton Nuclear Institute,The University of Manchester)for facilitating gamma sterilisation for our samples;financial support from Matoke Holdings。

摘　　要：Wounds pose a risk to the skin,our body's primary defence against infections.The rise of antibiotic resistance has prompted the development of novel therapies.RO-101^(■)is an antimicrobial gel that delivers therapeutic levels of hydrogen peroxide(H_(2)O_(2)),a reactive oxygen species,directly to the wound bed.In this study,electrospinning was used to incorporate RO-101^(■)into a polyvinyl alcohol(PVA)sub-micron fibrous mesh that can act as a delivery agent,achieve a sustained release profile,and provide a barrier against infection.Adequate incorporation of this gel into sub-micron fibres was confirmed via nuclear magnetic resonance spectroscopy.Furthermore,scanning electron microscopy exhibited smooth and uniform meshes with diameters in the 200-500 nm range.PVA/RO-101 electrospun meshes generated H_(2)O_(2) in concentrations exceeding 1 m M/(g·m L)(1 m M=1 mmol/L)after 24 h,and the role of sterilisation on H_(2)O_(2) release was evaluated.PVA/RO-101meshes exhibited antimicrobial activity against both Gram-positive Staphylococcus aureus(S.aureus)and Gram-negative Pseudomonas aeruginosa(P.aeruginosa)bacteria,achieving viable count reductions of up to 1 log unit CFU/mm^(2)(CFU:colony-forming units).Moreover,these meshes were capable of disrupting biofilm formation,even against multidrug-resistant organisms such as methicillin-resistant S.aureus(MRSA).Furthermore,increasing the RO-101^(■)concentration resulted in higher H_(2)O_(2) production and an enhanced antimicrobial effect,while fibroblast cell viability and proliferation tests showed a concentration-dependent response with high cytocompatibility at low RO-101^(■)concentrations.This study therefore demonstrates the potential of highly absorbent PVA/RO-101 meshes as potential antimicrobial wound dressings.

关 键 词：ANTIMICROBIAL ELECTROSPINNING Hydrogen peroxide Polyvinyl alcohol Reactive oxygen species Tissue engineering Wound dressing 

分 类 号：R318.08[医药卫生—生物医学工程]