药茶用菊花‘婺源皇菊’组培快繁体系研究  

Establishment of Tissue Culture and Rapid Propagation System of Medicinal and Tea Chrysanthemum‘Wuyuanhuangju’

作　　者：唐桂梅 肖晓玲 黄国林 刘洋 张力 欧阳瑶力 刘石峰 何涛 赵凡 TANG Guimei;XIAO Xiaoling;HUANG Guolin;LIU Yang;ZHANG Li;OUYANG Yaoli;LIU Shifeng;HE Tao;ZHAO Fan(Hunan Horticultural Research Institute,Hunan Academy of Agricultural Science,Changsha 410125,China;Yuelu Shan Laboratory,Changsha 410125,China;Hunan Provincial Key Laboratory of Germplasm Innovation and Comprehensive Utilization of Garden Flowers,Changsha 410125,China;Hunan Kangdejia Forestry Technology Co.,Yongzhou 425600,China;Hunan Yueningkang Biotechnology Co.,Yongzhou 425600,China)

机构地区：[1]湖南省农科院园艺研究所,湖南长沙410125 [2]岳麓山实验室,湖南长沙410125 [3]园林花卉种质创新与综合利用湖南省重点实验室,湖南长沙410125 [4]湖南省康德佳林业科技有限责任公司,湖南永州425600 [5]湖南省粤宁康生物科技有限公司,湖南永州425600

出　　处：《湖南生态科学学报》2024年第4期77-84,共8页Journal of Hunan Ecological Science

基　　金：湖南省林业生态保护修复及发展专项资金项目(湘财资环指[2023]5号、湘财资环指[2024]3号);湖南省重点研发项目(2021NK2008、2022NK2062);湖南省农业科技创新资金项目(2024CX30)。

摘　　要：【目的】本研究旨在建立‘婺源皇菊’的组培快繁体系,为规模化生产药茶用菊花‘婺源皇菊’种苗提供技术支撑。【方法】以‘婺源皇菊’幼嫩茎段为外植体,次氯酸钠为消毒剂,消毒时间分别为5(A1)、10(A2)、15(A3)和20(A4)min,以MS培养基为基本培养基,并添加不同浓度组合的植物生长调节剂,对外植体的消毒、初代培养、继代培养、生根培养、炼苗移栽过程进行了研究。【结果】不同消毒时间、不同植物生长调节剂配比对‘婺源皇菊’外植体初代培养效果影响显著。不同消毒时间处理对外植体褐化率和染菌率的影响分别为:A1<A2<A3<A4,A1>A2>A3>A4;初代培养时添加6-BA 2.0 mg/L+NAA 0.2 mg/L+KT 0.1 mg/L的培养基芽诱导率、出芽指数及苗高均为最高;继代增殖培养时则为添加6-BA 1.5 mg/L+NAA 0.2 mg/L+KT 0.1 mg/L的培养基芽诱导率和出芽指数最高;生根培养时添加6-BA 0.5 mg/L+NAA 0.3 mg/L+IBA 0.2 mg/L的组培苗生根率最高、单株平均根数最多、单株平均根长最长;最适宜的炼苗天数为6~7 d。【结论】外植体最佳消毒方式为70%酒精消毒10 s后,用2%次氯酸钠消毒10 min;以MS为基础培养基,初代培养最佳植物生长调节剂组合为6-BA 2.0 mg/L+NAA 0.2 mg/L+KT 0.1 mg/L,继代培养最佳组合为6-BA 1.5 mg/L+NAA 0.2 mg/L+KT 0.1 mg/L;生根培养的基本培养基为1/2MS,最佳植物生长调节剂组合为6-BA 0.5 mg/L+NAA 0.3 mg/L+IBA 0.2 mg/L。本研究初步构建了‘婺源皇菊’的组培快繁技术流程,为‘婺源皇菊’种苗的规模化繁殖提供了理论基础与技术支撑。【Objective】The aim of this study is to establish a fast breeding system of‘Wuyuanhuangju’,and to provide technical support for the large-scale production of‘Wuyuanhuangju’seedlings.【Method】The disinfection,primary culture,secondary culture,rooting culture and seedling transplantation process of‘Wuyuanhuangju’were studied by using young stem segments of‘Wuyuanhuangju’as the explant,hypochloric acid as the disinfectant,the disinfection times were 5(A1),10(A2),15(A3),and 20(A4)minutes,respectively,and different plant growth regulator combinations added with MS culture medium as the basic culture medium.【Result】Different sterilization time and different plant growth regulator ratios had significant effects on the primary culture effect of‘Wuyuanhuangju’explants.The ratios of browning and bacterial contamination of tissue culture seedling in different treatments caused by sterilization time were:A1<A2<A3<A4,A1>A2>A3>A4 respectively;The medium supplemented with 6-BA 2.0 mg/L+NAA 0.2 mg/L,and KT 0.1 mg/L had the highest bud induction rate,germination index,and seeding height during the initial cultivation;The medium supplemented with 6-BA 1.5 mg/L,NAA 0.2 mg/L,and KT 0.1 mg/L showed the highest bud induction rate and germination index during subculture;The tissue culture seedlings with added 6-BA 0.5 mg/L+NAA 0.3 mg/L+IBA 0.2 mg/L had the highest rooting rate,the highest average number of roots per plant,and the longest average root length per plant during rooting cultivation;the optimal number of days of seedling refining was 6~7 days.【Conclusion】The optimal disinfection method was 70%alcohol disinfection for 10 seconds,followed by disinfection with 2%hypochloric acid for 10 minutes;the optimal primary culture medium MS+6-BA 2.0 mg/L+NAA 0.2 mg/L+KT 0.1 mg/L;optimal successional medium MS+6-BA 1.5 mg/L+NAA 0.2 mg/L+KT 0.1 mg/L;optimal rooting medium 1/2MS+6-BA 0.5 mg/L+NAA 0.3 mg/L+IBA 0.2 mg/L.This study preliminarily constructed the tissue culture and rapid propagation technology p

关 键 词：‘婺源皇菊’ 药茶用菊 外植体 组培 快繁 

分 类 号：S567[农业科学—中草药栽培] Q94[农业科学—作物学]