红阳猕猴桃盐胁迫下生理生化响应及相关基因表达分析  

Physiological and biochemical response and related gene expression of Hongyang kiwiftuit seedlings under salt stress

作　　者：朱润洁 闻蒙蒙 郎红珊 段好鑫 李玥 汤晓丽 ZHU Runjie;WEN Mengmeng;LANG Hongshan;DUAN Haoxin;LI Yue;TANG Xiaoli(College of Horticulture,Ludong University,Yantai 264025,Shandong,China)

机构地区：[1]鲁东大学园艺学院,山东烟台264025

出　　处：《果树学报》2024年第11期2224-2234,共11页Journal of Fruit Science

基　　金：山东省自然科学基金面上项目(ZR2023MC200)。

摘　　要：[目的]探究不同浓度盐处理对红阳猕猴桃生理生化特性和相关基因表达的影响,揭示该品种猕猴桃的抗盐性。[方法]以红阳猕猴桃苗为试材,Na Cl浓度分别为0、50、100、150和200 mmol·L^(-1),处理10 d后测定植株的生理生化指标及盐胁迫相关基因的表达情况。[结果]随着盐浓度的升高,植株的净光合速率(P_(n))、过氧化氢酶(CAT)活性和维生素C(VC)含量显著下降,相对电导率(REC)、脯氨酸(Pro)含量和超氧化物歧化酶(SOD)活性显著上升,过氧化物酶(POD)活性呈先下降后上升趋势,可溶性糖、可溶性蛋白和丙二醛(MDA)含量呈现先上升后下降的趋势。q RT-PCR分析发现,盐胁迫下乙烯受体、褪黑素响应、ABA反馈调节、渗透胁迫响应和活性氧(ROS)生成基因被诱导表达;ABA分解代谢和VC合成基因的表达被抑制。[结论]红阳猕猴桃幼苗可有效调节100 mmol·L^(-1)及以下的Na Cl盐胁迫,试验结果为盐碱地种植猕猴桃提供了理论参考。【Objective】Kiwifruit is rich in vitamin C,dietary fiber and a variety of minerals,and has rich nutritional value.It is one of the most popular fruits in the world,but it is also a fruit crop that is sensitive to salt stress.The saline land in China has a wide distribution,and affects plant growth and soil permeability.It results in soil hardening and ion imbalance in plants,limiting the cultivation of kiwi-fruit seriously.Therefore,it is of great significance to study the salt tolerance of kiwifruit and solve the problem of kiwifruit cultivation in the saline land.In this research,physiological and biochemical re-sponse and related gene expression of Hongyang seedlings under salt stress were analyzed.【Methods】The seedlings under the same growth conditions were transplanted into a pure vermiculite culture dish and cultured in the greenhouse for 1 month.The method of NaCl treatment was to pour Hoagland nutri-ent solution every 3 days,and the NaCl concentrations of Hoagland nutrient solution were 0,50,100,150 and 200 mmol·L^(-1),respectively.The net photosynthesis rate(Pn)and relative conductivity(REC)was detected immediately after NaCl treatment.The antioxidant enzyme activities,the contents of os-motic adjustment substances,vitamin C(VC)and malondiadehyde(MDA)as well as the expressions of the salt-stress related genes were measured subsequently.【Results】Along with the increase of NaCl concentrations,the growth condition of Hongyang seedlings was gradually deteriorated and limited.Compared with the control,the plants of experimental groups were dwarfed and wilted,and the leaf edge became scorched.The treatments of 50 and 100 mmol·L^(-1) had relatively little effect on young seedlings.With 150 mmol·L^(-1) treatment,the plants began to show obvious yellowing,wilting and dwarfing.When the concentration achieved 200 mmol·L^(-1),the plants showed wilted and stunted serious-ly.Meanwhile,with the increasing of NaCl concentrations,the Pn decreased and the REC increased.The activity of peroxidase(POD)decreas

关 键 词：红阳猕猴桃 盐胁迫 生理生化指标 基因表达 

分 类 号：S663.4[农业科学—果树学]