毛花猕猴桃成熟期果实品质和蔗糖代谢特征分析  

Analysis of the fruit quality and the metabolic characteristics of sucrose in fruit of Actinidia eriantha at ripening stage

作　　者：贾东峰 钱基权 高欢 廖光联 钟敏[2] 黄春辉[1,2] 徐小彪[1,2] JIA Dongfeng;QIAN Jiquan;GAO Huan;LIAO Guanglian;ZHONG Min;HUANG Chunhui;XU Xiaobiao(College of Agronomy,Jiangxi Agricultural University,Nanchang 330045,Jiangxi,China;Institute of Kiwifruit,Jiangxi Agricultural University,Nanchang 330045,Jiangxi,China)

机构地区：[1]江西农业大学农学院,南昌330045 [2]江西农业大学猕猴桃研究所,南昌330045

出　　处：《果树学报》2024年第11期2261-2271,共11页Journal of Fruit Science

基　　金：国家自然科学基金项目(32160692,32460742);江西省自然科学基金项目(20242BAB25371)。

摘　　要：[目的]探究果实成熟期可溶性糖含量差异大的原因,以挖掘毛花猕猴桃果实蔗糖代谢关键酶和关键基因。[方法]以高糖型赣绿1号和低糖型赣绿6号毛花猕猴桃品种为研究材料,测定果实生理成熟期外观指标;测定软熟期可溶性固形物含量、干物质含量、可溶性糖含量、糖组分含量、甜度值、蔗糖代谢酶活性和代谢酶基因相对表达量等指标;分析不同指标间的相关性。[结果]果实成熟期可溶性糖含量与蔗糖含量呈显著正相关;蔗糖含量与蔗糖磷酸合酶活性及其编码基因AeSPS表达量呈显著正相关。[结论]蔗糖是引起毛花猕猴桃果实可溶性糖含量差异大的主要原因,蔗糖磷酸合酶是影响果实成熟期蔗糖代谢的关键酶,推测其编码基因AeSPS是调控果实成熟期蔗糖合成的关键基因。【Objective】Actinidia eriantha is a unique kiwifruit species of Actinidia spp.The fruit of A.eriantha is rich in nutrients and ascorbic acid(vitamin C).However,the flavor quality of most wild A.eriantha resources is lower than that of the cultivars from A.chinensis or A.deliciosa mainly due to the low accumulations of soluble sugars.Fortunately,a high sugar type kiwifruit cultivar,A.eriantha‘GanlüNo.1’,has been developed by our research group recently.In order to explore the formational mechanism of the high accumulation of soluble sugars,and to identify the related key metabolic en-zyme and key gene in the fruit of A.eriantha‘GanlüNo.1’at the ripening stage,A.eriantha‘GanlüNo.1’and another low sugar type kiwifruit cultivar,A.eriantha‘GanlüNo.6’,were selected as the re-search materials in this study.These two cultivars possess similar genetic background and they both originated from the wild A.eriantha resources of Magu Mountain in Nancheng County,Jiangxi Prov-ince.【Methods】The grafted vines of A.eriantha‘GanlüNo.1’and A.eriantha‘GanlüNo.6’were planted in an orchard of the Fengxin County Bureau of Agriculture and Rural Affairs,which was locat-ed in Fengxin County,Jiangxi Province.In 2021,at physiological maturity stage when the soluble sol-ids content of fruits of each cultivar reached over 7.0%,fruits without pests,diseases and mechanical damage were collected randomly from selected vines.After collection,the fruits were placed in an ice box and transported to the laboratory for further treatment.For each cultivar,three uniform vines were used as three biological replicates.Twenty fruits were collected from each vine.For fresh fruits,the fruit weight was measured using an electronic balance;the fruit longitudinal diameter and fruit trans-verse diameter were measured respectively with a vernier caliper.The fruit shape index was obtained us-ing the ratio of the longitudinal diameter to the transverse diameter.Then those fruits were stored at room temperature until soft-ripe sta

关 键 词：毛花猕猴桃 可溶性糖 蔗糖 糖代谢 蔗糖磷酸合酶 果实品质 

分 类 号：S663.4[农业科学—果树学]