猕猴桃溃疡病不同发病程度下花部微生物群落结构和多样性分析  

Microbial community structure and diversity of kiwifruit flowers infect-ed by different degrees of bacterial canker

作　　者：刘浩浩 钟彩虹[2] 刘巍[1] 李黎[2] 黄丽丽[1] LIU Haohao;ZHONG Caihong;LIUWei;LI Li;HUANG Lili(College of Plant Protection,Northwest A&F University,Yangling 712100,Shaanxi,China;Wuhan Botanical Garden,Chinese Acade-my of Sciences CAS Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture/CAS Engineering Laboratory for Kiwi-fruit Industrial Technology,Wuhan 430074,Hubei,China)

机构地区：[1]西北农林科技大学植物保护学院,陕西杨凌712100 [2]中国科学院武汉植物园·中国科学院植物种质创新与特色农业重点实验室·中国科学院猕猴桃产业技术工程实验室,武汉430074

出　　处：《果树学报》2024年第11期2323-2334,共12页Journal of Fruit Science

基　　金：国家重点研发项目(2022YFD1400200);湖北省第四批现代农业产业技术体系专项(2023HBSTX4-08);湖北省重点研发计划项目(2022BBA0076);国家现代农业产业技术体系(CARS-26);植物基因组学国家重点实验室开放课题(SKLPG2016A-18)。

摘　　要：[目的]探究不同发病程度对猕猴桃花部细菌和真菌微生物多样性及群落结构的影响,为猕猴桃溃疡病的生物防治提供一定的基础。[方法]以东红猕猴桃不同溃疡病发病程度(健康、中等、严重)的花组织为研究对象,运用高通量测序技术进行微生物组成和动态表征,探究溃疡病不同发病程度对猕猴桃花细菌和真菌微生物多样性及群落结构影响。[结果]随着发病程度的加剧,细菌微生物多样性下降;真菌微生物多样性先上升后下降。显著性研究结果表明,健康样本中优势细菌为蓝细菌、鞘氨醇单胞菌属,优势真菌为被孢霉属;中等发病程度样本中优势细菌为Escherichia Shigella,优势真菌为镰刀菌属、曲霉属、维希尼克氏酵母;严重发病程度样本中的优势细菌为假单胞菌属,优势真菌为链格孢属、枝孢属、线黑粉酵母属。[结论]综上可知,溃疡病菌的入侵显著改变了猕猴桃花的微生物群落结构。被孢霉属在健康样本中显著富集;链格孢属、枝孢属等菌属在严重发病程度样本中显著富集。该研究结果为猕猴桃-溃疡病病菌的互作机制研究及溃疡病的生物防治奠定了理论基础。【Objective】Kiwifruit canker is a severe bacterial disease that poses a significant threat to the kiwifruit industry causing by Pseudomonas syringae pv.actinidiae(Psa).This disease affects various parts of kiwifruit,including the vines,leaves,flowers and roots.Among these,the impact on the flowers is particularly critical,as it directly leads to yield loss in the affected year.Psa can damage the buds,petals and peels,causing the buds to fail to bloom,turn brown,and even fall off.Flower organs provide a conducive environment for microbial colonization.Consequently,microorganisms residing inside or on the surface of flower play a protective role against pathogen infection.Numerous studies have shown that environmental factors influence microbial community composition of plants.Among these factors,external pathogen invasion acts as the major selection pressure,significantly affecting floral microbial community structure.This study aimed to investigate changes in microbial diversity and community structure of floral bacteria and fungi under varying canker disease severity conditions,by using high-throughput sequencing.The results laid a theoretical foundation for understanding the interactive mechanisms between kiwifruit and Psa,as well as for developing biological control strate-gies for this disease.【Methods】In April and May,2022,during the flowering period of the Donghong kiwifruit,which was also a highly-infected period for kiwifruit canker disease,samples were collected from a kiwifruit experimental orchard in Xijiadian Town,Danjiangkou City,Hubei province.Three disease severity levels were set as below:healthy,moderate and severe,respectively.High-throughput sequencing technology was employed to analyze the diversity and structure of bacterial and fungal microflora in these samples.Specifically,16S rRNA and ITS1 gene sequencings were used to study bacterial and fungal communities,respectively.【Results】A total of 584580 high-quality 16S rRNA sequences and 520169 high-quality ITS1 sequences were obtain

关 键 词：猕猴桃溃疡病 微生物组 发病程度 群落组成 

分 类 号：S663.4[农业科学—果树学]