纳米颗粒表面蛋白冠的形成机制和表征技术研究进展  

Research progress of formation mechanism and characterization techniques of protein corona on the surface of nanoparticles

作　　者：张永杰 黄彬铜 翟月明 ZHANG Yongjie;HUANG Bintong;ZHAI Yueming(The Institute for Advanced Studies(IAS),Wuhan University,Wuhan 430072,China;School of Physics and Technology,Wuhan University,Wuhan 430072,China)

机构地区：[1]武汉大学高等研究院,武汉430072 [2]武汉大学物理与技术学院,武汉430072

出　　处：《无机化学学报》2024年第12期2318-2334,共17页Chinese Journal of Inorganic Chemistry

基　　金：国家重点研发计划(No.2021YFA0909900);中央高校基本科研业务费(No.2042024kf0012);中国博士后科学基金(No.2023M742689,GZB20230545)资助。

摘　　要：在生物体液环境中,纳米颗粒表面会快速结合蛋白质,形成蛋白冠,这会极大地改变其理化性质,并影响与生物系统的相互作用。理解蛋白冠的形成机制和动态变化有助于优化纳米颗粒的设计,提高纳米药物的靶向性和有效性,减少副作用。在这篇综述中,我们首先回顾了蛋白冠的研究进展,详细介绍了蛋白冠的形成机制、影响因素以及调控方法和现阶段表征技术,最后讨论了现阶段蛋白冠研究面临的挑战,并基于此提出展望,以期不断深化对蛋白冠的认识,推动纳米医学和生物技术的发展,拓展其应用范围。In biological fluids,proteins bind to the surface of nanoparticles to form a coating named the protein corona,which can dramatically change their physicochemical properties and affect interactions with living systems.Understanding the formation mechanism and dynamic changes of protein corona can help optimize the design of nanoparticles,improve the targeting and effectiveness of nanomedicines,and reduce side effects.In this review,we first reviewed the progress of protein corona and highlighted the properties of protein corona,the factors influencing their formation,and the analytical techniques for probing the protein corona.Finally,we share our perspective on the challenges and opportunities of protein corona in characterization methods and controlling their formation and composition.This review will contribute to understanding protein corona for the development and application of nanomedicine and biotechnology.

关 键 词：纳米颗粒 纳米-生物相互作用 蛋白冠 纳米无机-生物界面 表征方法 

分 类 号：O629.73[理学—有机化学]