绿农林■31复合微生物菌剂性能评价及其对海南大叶种茶茶饼病发生和叶片内生细菌群落的影响  

Evaluation of the Performance of Lvnonglin®31 Compound Bacterial Agent and Its Effect on the Occurrence of Blister Blight and Endophytic Bacterial Community in Leaves of Camellia sinensis var.assamica in Field

作　　者：周游[1] 李晓霞 刘建 邓涛 黄俊生[1] 杨腊英[1] 符红文 汪军[1] ZHOU You;LI Xiaoxia;LIU Jian;DENG Tao;HUANG Junsheng;YANG Laying;FU Hongwen;WANG Jun(Institute of Environment and Plant Protection,Chinese Academy of Tropical Agricultural Sciences/National Fertilizer Microbial Germplasm Resource Bank(Hainan),Haikou,Hainan 571101,China;Institute of Scientific and Technical Information,Chi-nese Academy of Tropical Agricultural Sciences,Haikou,Hainan 571101,China;Heze Customs,people’s Republic of China,Heze,Shandong 274000,China;Institute of Microbiology,Jiangxi Academy of Sciences,Nanchang,Jiangxi 330096,China;Hainan Baolvchun Agricultural Development Co.,Ltd.,Haikou,Hainan 571100,China)

机构地区：[1]中国热带农业科学院环境与植物保护研究所/国家肥料微生物种质资源库(海南),海南海口571101 [2]中国热带农业科学院科技信息研究所,海南海口571101 [3]中华人民共和国菏泽海关,山东菏泽274000 [4]江西省科学院微生物研究所,江西南昌330096 [5]海南宝绿春农业开发有限公司,海南海口571100

出　　处：《热带作物学报》2024年第12期2633-2645,共13页Chinese Journal of Tropical Crops

基　　金：江西省重点研发计划项目(No.20223BBF61015);海南省自然科学基金项目(No.322RC750,No.721RC631)。

摘　　要：探究绿农林■31复合微生物菌剂活性及其对海南大叶种茶茶饼病发生和茶叶内生细菌群落结构的影响,为合理应用微生物制剂绿色防控茶饼病提供理论依据。通过测试室内抗菌谱、激光共聚焦显微镜观察形态、测试病原真菌孢子萌发抑制率评价绿农林■31复合微生物菌剂(LNL31)活性,开展田间随机区组试验测试LNL31不同稀释倍数对海南大叶种茶茶饼病的防效,以及对海南大叶种茶茶树芽长、芽密度、鲜重和叶绿素的影响;采用16S rDNA测序技术,探究施用微生物菌剂条件下茶树叶部内生细菌群落结构的差异。结果表明:LNL31稀释液对坏损外担菌ER01、胶孢炭疽菌CG02、尖孢镰刀菌FOC4、柑橘黄单胞杆菌XC01、大肠杆菌DH5α5种病原菌的抑制作用明显,对ER01、CG02和FOC4的孢子萌发抑制率达89.04%~93.00%。田间施用LNL31500倍稀释液处理的防病促生作用最显著,与化学药剂吡唑醚菌酯处理相当,LNL311000倍稀释液次之。施药后14 d,LNL31500倍液、1000倍液对茶饼病的防效分别达91.98%和89.17%。LNL31对芽长、芽密度、鲜重和叶绿素的促生作用分别达23.14%~36.17%、32.10%~51.38%、27.66%~40.00%和28.88%~36.14%。喷施不同浓度的LNL31后,茶树叶部内生细菌群落的丰富度和均匀度均增加,主坐标分析(PCoA)结果表明,喷施LNL31对叶内细菌群落有显著影响;在目分类水平上,根瘤菌目(Rhizobiales)和鞘脂单胞菌目(Sphingomonadales)是较占优势的细菌目;在属分类水平上,甲基杆菌属(Methylobacterium)是最优势的内生细菌属,其次是1174_901_12、鞘氨醇单胞菌属(Sphingomonas)和根瘤菌属(Rhizobium);线性判别分析(LEfSe)结果显示,喷施LNL31的处理中具有9~14个指示菌群,而对照指示细菌属只有7个;物种丰度聚类分析表明,喷施LNL31的处理中独有优势属拜叶林克氏菌属(Beijerinckia)与伯克霍尔德菌属(Burkholderia)的丰度相似,根瘤菌属与1174_901_12的丰度相似�The purpose of this study is to explore the relationship between the activity of Lvnonglin■31 compound microbial agent(LNL31),the occurrence of blister blight and the structure of endophytic bacterial communities in tea,and to provide a theoretical basis for the rational application of microbial agents for green control of blister blight in Camellia sinensis var.assamica.The activity of LNL31 was evaluated through indoor antibacterial spectrum testing,laser confocal microscope morphology observation,pathogenic fungus spore germination inhibition rate test,and transparent circle detection enzyme activity methods.Field randomized block trials were carried out to test the disease prevention and growth promotion effects of LNL31 with different dilution ratios.Then the tea tree bud length,bud density,fresh weight and chlorophyll were respectively measured.Using 16S rDNA sequencing technology,the differences in the occurrence of the blister blight in C.sinensis var.assamica and the community structure of endophytic bacteria in leaves under LNL31 were investigated.The diluted LNL31 had obvious inhibitory effect on five pathogens,such as Exo-basidium vexans EV01,Colletotrichum gloeosporioides CG02,Fusarium oxysporum FOC4,Xanthomonas axonopodis pv.citri XC01 and Escherichia coli DH5α,which caused the hyphae to break,expand and digest.The inhibition rate of LNL31 on spore germination of pathogenic fungi EV01,CG02 and FOC4 reached 89.04%-93.00%.In field randomized block trials,the application of 500-fold dilution of LNL31 had the most significant disease prevention and growth promotion effect,which was similar to the treatment of the pyraclostrobin,followed by 1000-fold dilution of LNL31.The averages of bud length,germination density,hundred bud weight and chlorophyll of the plants in the treatment of LNL31 increased by 23.14%-36.17%,32.10%-51.38%,27.66%-40.00%and 28.88%-36.14%,respectively,in comparison with the control.After spraying different concentrations of LNL31,the richness and uniformity of endophytic bacterial

关 键 词：绿农林■31复合微生物菌剂 茶饼病 内生细菌 群落结构 

分 类 号：S31[农业科学—作物栽培与耕作技术]