宁夏枸杞多糖对UVB诱导HaCaT细胞光老化的保护作用及机制研究  

Study on the protective effect and mechanism of Ningxia lycium barbarum polysaccharide on photo-aging of UVB induced HaCaT cells

作　　者：雷鸣 高晓燕 刘瑞 姚守恩 孟灵杰 李伟宁[3] LEI Ming;GAO Xiaoyan;LIU Rui;YAO Shouen;MENG Lingjie;LI Weining(Traditional Chinese Medicine Hospital of Yinchuan,Yinchuan 750001,China;People′s Hospital of Ningxia Hui Autonomous Region,Yinchuan 750002,China;Affiliated Hospital of Shandong University of Traditional Chinese Medicine,Jinan 250000,China)

机构地区：[1]宁夏银川市中医医院,宁夏银川750001 [2]宁夏回族自治区人民医院,宁夏银川750001 [3]山东中医药大学附属医院,山东济南250000

出　　处：《宁夏医学杂志》2024年第11期921-924,I0001,共5页Ningxia Medical Journal

基　　金：陕西中医药大学校级科研课题(2020FS14);宁夏回族自治区青年拔尖人才培养工程项目(2020115);银川市科技创新领军人才培养项目(2023LJRC05);山东省中医药科技项目(M-2022155)。

摘　　要：目的 研究宁夏枸杞多糖(LBP)对中波紫外线(UVB)诱导人永生化角质形成细胞(HaCaT)光老化的保护作用及机制。方法 采用UVB刺激HaCaT细胞建立光老化细胞模型,噻唑蓝比色(MTT)法测定细胞活力,通过酶联免疫吸附试验(ELISA)检测白细胞介素-1β(IL-1β)、白细胞介素-6(IL-6)、人肿瘤坏死因子-α(TNF-α)的水平,并以其为指标,研究不同浓度的LBP对HaCaT细胞的抗光老化活性及其机制。结果 UVB照射HaCaT细胞后细胞增殖活性下降,LBP干预后照射组细胞活性上升,LBP含量为80μg/mL和160μg/mL时,细胞存活率分别为95.49%和100.00%。与空白对照组、UVB照射组相比,LBP浓度为160μg/mL时,IL-1β、IL-6、TNF-α水平均明显下降。结论 枸杞多糖有抗光老化作用,其机制可能是通过抑制UVB引起的HaCaT细胞增殖活性下降以及降低IL-1β、IL-6、TNF-α的表达水平来实现。Objective To study the protective effect and mechanism of Ningxia lycium barbarum polysaccharide(LBP)on the photoaging induced by medium-wave ultraviolet(UVB)light in human immortalized keratin-forming cells(HaCaT).Methods The photoaging cell model was established by stimulating HaCaT cells with UVB-induced.Cell viability was determined by the multiple table tournament(MTT)method.The levels of interleukin-1β(IL-1β),interleukin-6(IL-6),and human tumor necrosis factor-α(TNF-α)affected by enzyme-linked immunosorbent assay(ELISA)were detected as an index to study the effects of different concentrations of LBP on the anti-photo-aging activity of HaCaT cells and its mechanism.Results The proliferation activity of HaCaT cells decreased after UVB irradiation,and the cell activity of the irradiation group increased after intervention with lycium barbarum polysaccharide,and the cell survival rate was categorized into 95.49%and 100%when the LBP content was 80μg/mL and 160μg/mL..Compared to the blank control group and the UVB irradiation group,the LBP concentration was 160 g/mL,the levels of IL-1β,IL-6,and TNF-αwere decreased significantly.Conclusion LBP has an anti-photoaging effects,the mechanism may be through inhibiting the proliferation activity of HaCaT cells by UVB induced and reducing the expression levels of IL-1β,IL-6 and TNF-α.

关 键 词：宁夏枸杞多糖 中波紫外线 人永生化角质形成细胞 光老化 保护作用 

分 类 号：R285[医药卫生—中药学]