原发性干燥综合征合并焦虑患者口腔菌群分析  

Analysis of oral flora in patients with primary Sjogren′s syndrome complicated with anxiety

作　　者：杜鹃 朱瑜 宋宇航 谢戬芳[1] DU Juan;ZHU Yu;SONG Yuhang;XIE Jianfang(Department of Rheumatology and Immunology,Second Hospital of Shanxi Medi-cal University,Taiyuan 030000,China)

机构地区：[1]山西医科大学第二医院风湿免疫科,太原030000

出　　处：《山西医科大学学报》2024年第11期1465-1473,共9页Journal of Shanxi Medical University

摘　　要：目的 探讨原发性干燥综合征(primary Sjogren's syndrome,pSS)合并焦虑患者口腔菌群结构改变情况。方法 选择2023年10月至2024年3月山西医科大学第二医院风湿免疫科住院部60例pSS患者,根据汉密顿焦虑量表(Hamilton Anxiety Scale,HAMA)评分将患者分为pSS不伴焦虑组(pSS without anxiety,NASS)和pSS合并焦虑组(pSS with anxiety,ASS),两组纳入的患者各20例,选择同院健康护工20例为正常对照组(normal control,NC),采集所有受试者龈上或者表面牙菌斑,通过高通量测序检测细菌16S-rRNA序列,进行口腔微生物群落结构分析。结果 Alpha多样性比较中,三组间的Shannon指数、Simpson指数、Chao指数、Coverage指数差异均有统计学意义(P<0.05)。在门水平,NASS组厚壁菌门的相对丰度水平高于NC组,ASS组放线菌门、奇异球菌门的相对丰度水平高于NC组,ASS组放线菌门的相对丰度水平明显高于NASS组,差异均有统计学意义(P<0.05);在属水平,NASS组和ASS组韦荣氏球菌属、乳杆菌属相对丰度均高于NC组,ASS组放线菌属的相对丰度明显高于NASS组,差异均有统计学意义(P<0.05)。三组菌群影响因子(linear discriminant analysis effect size,LEfSe)分析显示,NC组菌群以口腔纤毛菌为主(P<0.05);NASS组以韦荣氏球菌科、韦荣氏球菌属为主(P<0.05);ASS组以放线菌科、放线菌属、乳杆菌科、乳杆菌属为主(P<0.05)。结论 pSS合并焦虑的患者与pSS不伴焦虑的患者以及正常人群的菌群结构在门和属水平上存在差异。放线菌科、放线菌属、乳杆菌科、乳杆菌属可能对pSS合并焦虑的发生发展起到重要作用。Objective To investigate the changes of oral flora composition in patients with primary Sjogren's syndrome(pSS)compli-cated with anxiety.Methods A total of 60 patients with pSS in the inpatient department of rheumatology and immunology of Second Hospital of Shanxi Medical University from October 2023 to March 2024 were collected.The patients were divided into pSS without anxiety(NASS)group and pSS with anxiety(ASS)group according to the Hamilton Anxiety Scale(HAMA),with 20 patients in each group.Twenty health care workers from the same hospital were selected as normal controls(NC group).Gingival or surface dental plaques of all subjects were collected,the bacterial 16S-rRNA sequence was detected by high-throughput sequencing,and the oral mi-crobial community composition was analyzed.Results For the Alpha diversity,the differences in Shannon index,Simpson index,Chao index,and Coverage index were statistically significant among the three groups(P<0.05).At the phylum level,the relative abun-dance of Firmicutes in NASS group was higher than that in NC group,the relative abundance of Actinobacteriota,Deinococcota in ASS group was higher than that in NC group(P<0.05),and the relative abundance of Actinobacteriota in ASS group was significantly higher than that in NASS group(P<0.05).At the genus level,the relative abundance of Veillonella and Lactobacillus was higher in NASS group and ASS group than that in NC group(P<0.05),and the relative abundance of Actinomyces in ASS group was significantly higher than that in NASS group(P<0.05).LEfSe analysis of bacteria between the three groups showed that Leptotrichia buccalis was predominant in NC group(P<0.05),Veillonellaceae and Veillonella were predominant in NASS group(P<0.05),and Actinomyceta-ceae,Actinomyces,Lactobacillaceae and Lactobacillus were predominant in ASS group(P<0.05).Conclusion The flora composi-tion of pSS patients without anxiety,pSS patients with anxiety and normal controls is different at the phylum and genus levels.Actino-mycetaceae,Actinomyces,Lactobaci

关 键 词：原发性干燥综合征 焦虑 口腔菌群 相关性分析 发病机制 菌群失调 

分 类 号：R593[医药卫生—内科学]