过敏性鼻炎患者血清Th17/Treg值与YKL-40及维生素D水平的相关性  

Correlations between serum Th17/Treg ratio and YKL-40 and vitamin D levels in patients with allergic rhinitis

作　　者：李红超 王昕磊 刘卫星 李静 LI Hongchao;WANG Xinlei;LIU Weixing;LI Jing(Department of Medical Laboratory,Tangshang Municipal Hospital of Traditional Chinese Medicine,Tangshan 063000,China;不详)

机构地区：[1]唐山市中医医院医学检验科,河北唐山063000 [2]唐山市中医医院康复科

出　　处：《山东医药》2024年第34期11-14,共4页Shandong Medical Journal

基　　金：河北省中医药类科学研究课题计划项目(2023406)。

摘　　要：目的探讨过敏性鼻炎患者血清辅助性T细胞17(Th17)/调节性T细胞(Treg)值与血清甲壳质酶蛋白-40(YKL-40)水平及维生素D水平的相关性。方法选择过敏性鼻炎患者99例(观察组)及同期体检健康者99例(对照组),采集空腹外周静脉血9 m L,采用酶联免疫吸附试验检测外周血25(OH)D_(3),采用流式细胞术检测外周血CD4^(+)IL-17+Th17细胞、CD4^(+)CD25^(+)Foxp3^(+)Treg细胞占CD4^(+)T细胞的百分比,采用双抗体夹心酶联免疫吸附法测定血清YKL-40,比较两组CD4^(+)IL-17+Th17细胞、CD4^(+)CD25^(+)Foxp3^(+)Treg细胞占CD4^(+)T细胞的百分比、YKL-40及维生素D水平。结果观察组血清25(OH)D_(3)水平和CD4^(+)CD25^(+)Foxp3^(+)Treg细胞占CD4^(+)T细胞的百分比低于对照组(P均<0.05),CD4^(+)IL-17+Th17细胞占CD4^(+)T细胞的百分比、Th17/Treg值及血清YKL-40水平高于对照组(P均<0.05)。观察组血清Th17/Treg值与YKL-40水平呈正相关(r=0.429,P<0.05),与血清25(OH)D_(3)水平呈负相关(r=-0.735,P<0.05);血清YKL-40与25(OH)D_(3)水平呈负相关(r=-0.450,P<0.05)。结论过敏性鼻炎患者血清25(OH)D_(3)水平降低,YKL-40水平升高,二者异常表达可能与Th17/Treg值失调有关,共同参与过敏性鼻炎的发生发展。Objective To investigate the relationships between the ratio of T helper cells 17(Th17)/regulatory T(Treg)cells and serum chitinase protein-40(YKL-40)level and vitamin D levels in patients with allergic rhinitis.Meth⁃ods Ninety-nine patients with allergic rhinitis(observation group)and 99 healthy individuals who underwent physical examination at the same time(control group)were selected.Peripheral venous blood 9 mL was collected,and the level of 25(OH)D_(3) in peripheral blood was detected by enzyme-linked immunosorbent assay.The proportion of CD4^(+)IL-17+Th17 cells and CD4^(+)CD25^(+)Foxp3^(+)Treg cells in CD4^(+)T cells was determined by flow cytometry.The level of YKL-40 in serum was measured by double antibody sandwich enzyme-linked immunosorbent assay.The proportions of CD4^(+)IL-17+Th17 cells,CD4^(+)CD25^(+)Foxp3^(+)Treg cells in CD4^(+)T cells,YKL-40 level and vitamin D level were compared between the two groups.Results The level of 25(OH)D_(3) in serum and the proportion of CD4^(+)CD25^(+)Foxp3^(+)Treg cells in CD4^(+)T cells were lower in the observation group than in the control group(both P<0.05),while the proportion of CD4^(+)IL-17+Th17 cells in CD4^(+)T cells,Th17/Treg cell ratio and serum YKL-40 level were higher in the observation group than in the control group(all P<0.05).The Th17/Treg ratio in serum was positively correlated with YKL-40 level(r=0.429,P<0.05)and was negatively correlated with the level of 25(OH)D_(3) in serum(r=–0.735,P<0.05).The level of YKL-40 was negatively correlated with the level of 25(OH)D_(3) in serum(r=–0.450,P<0.05).Conclusions In allergic rhinitis patients,serum 25(OH)D_(3) content decreases and YKL-40 level increases.The abnormal expression of YKL-40 may be related to the imbalance of Th17/Treg ratio and participate in the development and progression of allergic rhinitis.

关 键 词：过敏性鼻炎 辅助性T细胞17 调节性T细胞 血清甲壳质酶蛋白-40 维生素D 

分 类 号：R765.21[医药卫生—耳鼻咽喉科]