基于血液流变学及CGRP研究牡丹皮改善围绝经期综合征大鼠潮热的作用机制  

Mechanism of peony skin for improving hectic fever in rats with perimenopause syndrome based on hemorheology and calcitonin gene related peptide

作　　者：陈雯佳 王婕 王颖峥 王英豪[1] 黄美霞[1] Chen Wenjia;Wang Jie;Wang Yingzheng;Wang Yinghao;Huang Meixia(School of Pharmacy,Fujian University of Traditional Chinese Medicine,Fuzhou 350122,China)

机构地区：[1]福建中医药大学药学院,福州350122

出　　处：《国际医药卫生导报》2024年第23期3946-3950,共5页International Medicine and Health Guidance News

基　　金：国家中医药管理局临床中药学高水平中医药重点学科建设项目(国中医药人教函[2022]226号);福建省自然科学基金(2023J01857);2023年省直属单位教育科研专项资金资助(X2023005)。

摘　　要：目的基于血液流变学指标及降钙素基因相关肽(CGRP),初步探讨牡丹皮改善围绝经期综合征大鼠潮热的作用机制。方法研究时间为2022年9月至12月,研究地点为福建中医药大学实验动物中心,研究类型为基础研究。将40只雌性大鼠分为假手术组、模型组、牡丹皮给药组、清骨散阳性药组,每组10只。除假手术组外,其余大鼠采用去卵巢手术,术后第7天给予L-甲状腺素(92 mg/kg),每天1次,连续给药7 d,建立围绝经期综合征肾阴虚证“病证结合”模型。假手术组和模型组给予等量蒸馏水,牡丹皮给药组和清骨散阳性药组分别灌胃给予牡丹皮(2.2 g/kg)、清骨散(7.3 g/kg),每日1次,连续给药14 d。末次给药后1 h,麻醉大鼠、取血,检测血液流变学、ELISA法检测大鼠血清CGRP,实时荧光定量PCR法检测大鼠下丘脑CGRP的受体活性修饰蛋白1(RAMP1)、降钙素受体样受体(CLR)mRNA的表达情况。统计学方法采用单因素方差分析。结果模型组大鼠红细胞沉降率、血细胞压积、全血黏度(低切、中切、高切)等血液流变学指标,以及血清CGRP含量、下丘脑RAMP1、CLR mRNA表达水平均高于假手术组(均P<0.05),并被牡丹皮显著逆转,差异均有统计学意义(均P<0.05)。结论牡丹皮可以恢复围绝经期大鼠CGRP及其受体水平,改善血液流变学指标水平。Objective To explore the mechanism of peony skin for improving hectic fever in rats with perimenopause syndrome based on hemorheology indicators and calcitonin gene related peptide(CGRP).Methods From September to December 2022,the basic research was did at Experimental Animal Center,Fujian University of Traditional Chinese Medicine.Forty female rats were divided into a sham operation group,a model group,a peony skin group,and a Qinggusan-positive group,with 10 in each group.Except for the sham operation group,the other 3 groups underwent bilateral ovariectomy;L-thyroxine(92 mg/kg)was given once a day,for 7 consecutive days starting on the 7th day after the operation to establish Yin-deficient perimenopause syndrome models.The sham operation group and the model group were orally administered an equal amount of distilled water;the peony skin group and the Qinggusan-positive group were orally administered peony skin(2.2 g/kg)and Qinggusan(7.3 g/kg),respectively,once a day,for a total of 14 d.One hour after the last administration,the rats were anesthetized,and their blood was collected;the hemorheology indicators were detected;the serum CGRP was detected by enzyme-linked immunosorbent assay(ELISA);the expression of receptor activity-modifying protein 1(RAMP1)and calcitonin receptor-like receptor(CLR)mRNA in the rats'hypothalamuses were detected by reverse transcription-polymerase chain reaction(RT-PCR).The one-way analysis of variance was used for the statistical analysis.Results The erythrocyte sedimentation rate,hematocrit,low-,medium-,and high-shear whole blood viscosities,content of serum CGRP,and the expression levels of hypothalamic RAMP1 and CLR mRNA in the model group were higher than those in the sham operation group(all P<0.05),and were significantly reversed by peony skin,with statistical differences(all P<0.05).Conclusion Peony skin can improve the levels of CGRP,its receptor,and hemorheology in rats with perimenopause syndrome.

关 键 词：牡丹皮 围绝经期 潮热 血液流变学 降钙素基因相关肽 

分 类 号：R285.5[医药卫生—中药学]