大麻素受体1在肝星状细胞活化中的作用及蒿鳖养阴软坚方的干预效应  

Research on the role of cannabinoid receptor 1 in hepatic stellate cell activation and interfering effects of Haobie Yangyin Ruanjian

作　　者：杜岩 阮建佳 Du Yan;Ruan Jianjia(Tianjin Jizhou District People's Hospital,Tianjin 301900)

机构地区：[1]天津市蓟州区人民医院,天津301900

出　　处：《天津药学》2024年第5期17-20,共4页Tianjin Pharmacy

摘　　要：目的:研究大麻素受体1(CBR1)在肝星状细胞活化中的作用及蒿鳖养阴软坚方的干预效应。方法:将具有永生化特征的人源肝星状细胞LX-2作为研究对象,实验分为LX-2细胞对照组、蒿鳖养阴软坚方组、CBR1拮抗剂(AM251)组和AM251+蒿鳖养阴软坚方合用组。每组7个复孔,作用24 h,分别收集各实验组的LX-2细胞及培养液,按实验设计的检测方法进行检测。选用MTT法对LX-2的增殖情况进行检测;Western blot法检测CBR1和α-SMA蛋白表达情况;酶消化法测定羟脯氨酸(Hyp)的含量。结果:与细胞对照组比较,蒿鳖养阴软坚方及AM251均可显著减少CBR1和α-SMA蛋白的表达,抑制LX-2细胞的增殖,减弱Hyp的分泌(P<0.05);与AM251单用组比较,合用蒿鳖养阴软坚方可增强AM251对LX-2细胞增殖、CBR1和α-SMA蛋白的表达和Hyp分泌的抑制作用,两药有协同作用(P<0.05)。结论:蒿鳖养阴软坚方可通过干预大麻素受体信号通路抑制肝星状细胞的活化与增殖。Objective:To explore the role of cannabinoid receptor1 in the activation of hepatic stellate cells(HSCs)and interfering effects of Haobie Yangyin Ruanjian.Methods:Human hepatic stellate cells LX-2 with immortalized characteristics were used as the research object,and the experiment was divided into LX-2 cell control group,HBYYRJ group,CBR1 antagonist group(AM251),AM251 and HBYYRJ combination group.Each group consists 7 compound holes and was treated for 24 hours,LX-2 cells and culture medium from each experimental group are collected and tested according to the experimental design.MTT assay was adopted to measure the rate of proliferation of LX-2 cells.The expressions of CBR1 and α-SMA were evaluated by Western blot,Enzyme digestion method was used to detect the content of hydroxyproline(Hyp).Results:Compared with the control group,Haobie Yangyin Ruanjian(HBYYRJ)and AM251 could significantly reduce the expressions of CBR1 andα-SMA protein,inhibit the proliferation of LX-2 cells and weaken the secretion of hydroxyproline(Hyp)(P<0.05).Compared with the AM251 group,the combination of the two drugs,HBYYRJ could enhance the effects of CBR1 antagonist AM251 on the proliferation of LX-2 cells,the expression of CBR1 andα-SMA protein and the secretion of Hyp,and the two drugs had synergistic effect(P<0.05).Conclusions:Haobie Yangyin Ruanjian can inhibit the activation and proliferation of hepatic stellate cells by interfering with cannabinoid receptor signaling pathway.

关 键 词：肝纤维化 蒿鳖养阴软坚方 肝星状细胞 大麻素受体1 

分 类 号：R285[医药卫生—中药学]