灰葡萄孢培养基种类和继代培养对松材线虫致病力的影响  

Influence of Botrytis cinerea culture mediums and subculturing on the virulence of Bursaphelenchus xylophilus

作　　者：王道涵 瞿红叶[1] 米仁军 谈家金[1] WANG Daohan;QU Hongye;MI Renjun;TAN Jiajin(Co-Innovation Center for Sustainable Forestry in Southern China,College of Forestry and Grassland,Nanjing Forestry University,Nanjing 210037,China;Forestry Bureau of Chenxi County,Hunan Province,Huaihua 419599,China)

机构地区：[1]南京林业大学林草学院,南方现代林业协同创新中心,南京210037 [2]湖南省辰溪县林业局,怀化419599

出　　处：《植物保护》2024年第6期106-111,125,共7页Plant Protection

基　　金：国家重点研发计划(2021YFD1400900)。

摘　　要：本试验在8种培养基上培养灰葡萄孢Botrytis cinerea,以灰葡萄孢喂饲松材线虫Bursaphelenchus xylophilus,采用3年生黑松Pinus thunbergii作为接种材料,研究松材线虫繁殖力和致病力的变化,同时探究了在PDA-灰葡萄孢上继代培养84代的松材线虫致病力的变化。结果表明,以玉米、小麦和PDA 3种培养基培养的灰葡萄孢喂饲松材线虫,其繁殖力显著高于CDA和4种合成培养基,其中PDA-灰葡萄孢上的松材线虫数量最多,比合成培养基C或D培养的灰葡萄孢上的线虫数量提升了48.94%;接种PDA-灰葡萄孢组松材线虫的黑松病情指数最高,较最低的合成培养基D-灰葡萄孢组高了47.87%。松材线虫在PDA-灰葡萄孢上继代培养至第14代后,继代次数的增加会导致其致病力下降,接种第79代松材线虫后黑松病情指数已为0。因此,为保证松材线虫的致病力不衰退,应使用PDA-灰葡萄孢培养基喂饲松材线虫,且松材线虫继代培养应控制在14代以内。研究结果将为松材线虫的繁殖技术与致病相关机理分析等提供参考。In this study,changes in the fecundity and virulence of Bursaphelenchus xylophilus fed on Botrytis cinerea growing on eight different mediums and changes in the virulence of B.xylophilus subcultured on PDA for 84 generations were examined,using 3-year-old Pinus thunbergii as the inoculum material.The results showed that the fecundity of B.xylophilus fed on B.cinerea growing on three mediums,namely corn,wheat,and PDA,was significantly higher than those fed on B.cinerea on CDA and four synthetic mediums.The population size of B.xylophilus was highest on the combination of PDA and B.cinerea,48.94%higher than those the combination of synthetic medium C/D and B.cinerea.The disease index of P.thunbergii inoculated with B.xylophilus fed on B.cinerea on PDA was also the highest,47.87%higher than inoculation with B.xylophilus fed on B.cinerea on the synthetic medium D.However,after 14 generations of subculturing B.xylophilus fed on B.cinerea on PDA,an increase in successive generations led to a decrease in virulence.By the 79th generation,the disease index of P.thunbergii inoculated with B.xylophilus was zero.Therefore,to maintain virulence,B.xylophilus should be cultured on B.cinerea growing on PDA and subcultured for no more than 14 generations.These findings provide valuable insights into the reproduction technology and pathogenic mechanisms of B.xylophilus.

关 键 词：松材线虫 繁殖力 致病力 培养基 继代培养 

分 类 号：S763.7[农业科学—森林保护学]