海南省哈密瓜根结线虫病原种类鉴定  

Species identification of root-knot nematodes infecting Cucumis melo var.saccharinus in Hainan province

作　　者：王孟萍 赵晶 许环茹 丁晓帆 刘铜 WANG Mengping;ZHAO Jing;XU Huanru;DING Xiaofan;LIU Tong(Key Laboratory of Green Prevention and Control of Tropical Diseases and Pests,Ministry of Education,School of Tropical Agriculture and Forestry,Hainan University,Haikou 570228,China;Sanya Institute of Breeding and Multiplication,Hainan University,Sanya 572025,China)

机构地区：[1]热带农林生物灾害绿色防控教育部重点实验室,海南大学热带农林学院,海口570228 [2]海南大学三亚南繁研究院,三亚572025

出　　处：《植物保护》2024年第6期112-117,132,共7页Plant Protection

基　　金：海南省自然科学基金青年基金(323QN199)。

摘　　要：为明确海南省哈密瓜根结线虫病的发生情况及病原种类,2023年4月在海南哈密瓜主产区进行随机调查采样,并通过形态学和分子生物学技术对病原线虫种类进行鉴定。结果表明,哈密瓜根结线虫病在海南4个市县的11个调查点均有发生,其中东方好瑞村4-1采样点植株发病最严重,根结等级为5级。通过2龄幼虫形态测量和雌虫会阴花纹形态比对,结合线虫28S rDNA D2D3区段序列和SCAR特异性引物鉴定,最终鉴定出南方根结线虫Meloidogyne incognita、花生根结线虫M.arenaria和象耳豆根结线虫M.enterolobii,其中南方根结线虫在8个调查点有检出,个别点位出现南方根结线虫混合象耳豆根结线虫或花生根结线虫的情况。南方根结线虫为海南省哈密瓜上的主要病原线虫种类。In order to explore the occurrence of the root-knot nematode disease of Cucumis melo var.saccharinus and understand the species of nematode in Hainan province,the disease occurrence was investigated and the pathogens from samples randomly collected from the main producing area in Hainan in April,2023 were identified using morphology and molecular biology methods.The results showed that the root-knot nematode disease occurred in all the 11 investigated places,and nematode disease was most severe at sampling point 4-1 in Haorui village,Dongfang city,with root-knot grade of five.Meloidogyne incognita,M.arenaria and M.enterolobii were identified based on morphological characteristics of second-stage juveniles and female perineal pattern in combination with 28S rDNA D2D3 and SCAR sequences.M.incognita was the most widespread and was detected at eight sampling sites.Mixed populations of M.incognita and M.enterolobii or M.incognita and M.arenaria were happened.The above results indicate that M.incognita is the dominant population of root-knot nematode,which will provide a theoretical basis for the effective control of root-knot nematodes on Cucumis melo var.saccharinus in Hainan province.

关 键 词：哈密瓜 根结线虫 种群鉴定 海南省 

分 类 号：S432.45[农业科学—植物病理学]