云南常见马铃薯品种对马铃薯金线虫的抗性及其与抗性基因H1的相关性  

The correlations between the resistance of common potato varieties to Globodera rostochiensis and the H1 gene in Yunnan province

作　　者：段锦凤 杨艳梅 刘玉竹 李乾坤 胡先奇 DUAN Jinfeng;YANG Yanmei;LIU Yuzhu;LI Qiankun;HU Xianqi(State Key Laboratory for the Conservation and Utilization of Biological Resources in Yunnan,College of Plant Protection,Yunnan Agricultural University,Kunming 650201,China)

机构地区：[1]云南农业大学植物保护学院,省部共建云南生物资源保护与利用国家重点实验室,昆明650201

出　　处：《植物保护》2024年第6期126-132,共7页Plant Protection

基　　金：云南省基础研究专项-青年项目(202301AU070116);云南省农业联合专项-面上项目(202301BD070001-043);2019年中央农业生产救灾(第十批)资金课题(云财农[2020]7号)。

摘　　要：为明确云南省常见马铃薯品种对马铃薯金线虫Globodera rostochiensis抗性的差异,选择云南省常见或生产上主要推广的马铃薯品种25个,参考Koroleva的评价方法,结合抗性分子标记N146、N195、57R、TG432、Gro1-4-1和抗性基因H1的相对表达量,评价马铃薯品种对金线虫的抗性,分析抗性与抗性基因H1表达量的相关性。结果显示:供试的25个品种中,有7个品种根系上马铃薯金线虫的孢囊或雌虫≤5个,表现抗性,其他品种根系上的孢囊或雌虫大于5个,表现为易感;抗性分子标记检测发现,有10个品种含有抗马铃薯金线虫基因H1;对这10个品种的H1基因相对表达量的检测结果显示,接种马铃薯金线虫后‘V7’‘黄金薯’‘会薯19号’‘宣薯5号’‘云薯202’‘云薯304’‘云薯505’7个品种的H1基因较未接种时上调表达,而‘P14187’‘会薯15号’‘希森3号’3个品种的H1基因下调表达,携带H1基因的马铃薯品种是否对金线虫有抗性与抗性基因H1表达量呈正相关。研究结果为抗金线虫马铃薯品种选育与应用及进一步阐明马铃薯抗金线虫的分子机制奠定了基础。To identify the differences in resistance among common potato varieties to Globodera rostochiensis in Yunnan province,25 varieties,including both common and widely cultivated ones,were selected for evaluation using Koroleva’s method.In this study the molecular markers N146,N195,57R,TG432,Gro1-4-1,and the H1 resistance gene were employed to evaluate resistance levels and analyze the correlation between resistance and H1 gene expression.The results showed that among the 25 varieties tested,seven varieties exhibited resistance,with less than five cysts or females of G.rostochiensis on their root systems,while the remaining varieties,with more than five cysts or females,were susceptible.Molecular marker analysis revealed that ten varieties containing the G.rostochiensis resistance gene H1.Further investigation into the relative expression of the H1 gene after inoculation with G.rostochiensis showed that in seven varieties,namely‘V7’‘Huangjinshu’‘Huishu 19’‘Xuanshu 5’‘Yunshu 202’‘Yunshu 304’,and‘Yunshu 505’,the H1 gene expression was up-regulated compared to uninoculated controls.However,in three varieties‘P14187’‘Huishu 15’and‘Xisen 3’,the H1 gene expression was down-regulated.The study concluded that the resistance of potato varieties carrying the H1 gene were positively correlated with H1 gene expression.These findings provide foundation for selecting and applying potato varieties resistant to G.rostochiensis and for further elucidating the molecular mechanisms underlying potato resistance to G.rostochiensis.

关 键 词：马铃薯品种 马铃薯金线虫 H1基因 抗性 

分 类 号：S435.32[农业科学—农业昆虫与害虫防治]