风湿定胶囊中徐长卿和白芷质量控制研究  

Study on Quality Control of Cynanchum Paniculatum and Angelica in Fengshiding Capsule

作　　者：刘冬兰 郭璐玫 程韬[1] 杨洺 Liu Donglan;Guo Lumei;Cheng Tao;Yang Ming(Guizhou Institute for Food and Drug Control,Guiyang 550004,China)

机构地区：[1]贵州省食品药品检验所,贵州贵阳550004

出　　处：《亚太传统医药》2024年第8期49-54,共6页Asia-Pacific Traditional Medicine

基　　金：国家药品抽检项目(2021年度)。

摘　　要：目的:研究风湿定胶囊中徐长卿和白芷的质量控制方法。方法:采用薄层色谱法对风湿定胶囊中徐长卿和白芷进行鉴别研究,采用高效液相色谱法测定风湿定胶囊中徐长卿中丹皮酚和白芷中欧前胡素、异欧前胡素的含量,色谱柱waters Xbridge C_(18)(250 mm×4.6 mm,5μm),流动相乙腈-2%冰乙酸(50∶50)溶液,流速1.0 mL/min,柱温30℃,检测波长250 nm(欧前胡素、异欧前胡素)和274 nm(丹皮酚)。结果:薄层色谱斑点清晰,分离度较好;采用高效液相色谱同时测定欧前胡素、异欧前胡素、丹皮酚,分别在0.0106μg~0.2128μg、0.0031μg~0.0620μg、0.0147μg~1.4660μg范围内线性关系良好;平均回收率分别为100.0%(RSD=0.5%)、101.2%(RSD=0.9%)、98.9%(RSD=1.1%)。结论:该方法准确可靠,重复性好,可用于风湿定胶囊中徐长卿和白芷的质量控制。Objective:To study the quality control method of Cynanchum Paniculatum and Angelica in Fengshiding capsule.Methods:The identification of Cynanchum Paniculatum and Angelica angelica in Fengshiding capsule was studied by thin Layer chromatography.The contents of paeonol of Cynanchum Paniculatum and imperonin and isoimperonin of Angelica Angelica in Fengshiding capsule were determined by high performance Liquid chromatography(HPLC).The chromatographic column was waters Xbridge C_(18)(250 mm×4.6 mm,5μm).The mobile phase was acetonitrile-2%glacial acetic acid(50∶50)solution,the flow rate was 1.0mL/min,the column temperature was 30℃,and the detection wavelengths were 250nm(imperatorin,isoimperatorin)and 274nm(paeonol).Results:TLC spots were clear and the separation degree was good.The linear relationship of simultaneous determination of imperatorin,isoimperatorin and paeonol was good in 0.0106μg-0.2128μg,0.0031μg-0.0620μg,0.0147μg-1.4660μg by HPLC.The average recoveries were 100.0%(RSD=0.5%),101.2%(RSD=0.9%)and 98.9%(RSD=1.1%),respectively.Conclusion:The method is accurate,reliable and reproducible,and can be used for the quality control of Cynanchum Paniculatum and Angelica in Fengshiding capsule.

关 键 词：风湿定胶囊 丹皮酚 欧前胡素 异欧前胡素 质量控制 

分 类 号：R284.1[医药卫生—中药学]