通脉乐口服液中黄芪和丹参成分薄层色谱鉴别方法  

TLC Method for Identification of Astragalus and Salvia Miltiorrhiza Components in Tongmaile Oral Liquid

作　　者：李秋萍 李倩 张蓓 谭柳萍 陶晓静 张新林 张洪平 陆世银 Li Qiuping;Li Qian;Zhang Bei;Tan Liuping;Tao Xiaojing;Zhang Xinlin;Zhang Hongping;Lu Shiyin(Pharmacy Department,Liuzhou Traditional Chinese Medicine Hospital(Liuzhou Zhuang Medical Hospital)/Liuzhou Key Laboratory for Preparation Development of Chinese Materia Medica(Zhuang and Yao)/Liuzhou Engineering Research Center for Preparation Technology of Chinese Materia Medica(Zhuang and Yao),Liuzhou 545001,China;Guangxi University of Chinese Medicine,Nanning 530200,China)

机构地区：[1]柳州市中医医院(柳州市壮医医院)药学部,柳州市中药(壮瑶药)制剂研发重点实验室,柳州市中药(壮瑶药)制剂开发工程技术研究中心,广西柳州545001 [2]广西中医药大学,广西南宁530200

出　　处：《亚太传统医药》2024年第8期65-68,共4页Asia-Pacific Traditional Medicine

基　　金：广西壮族自治区中医药管理局科技项目(GZZC2020421);广西壮族自治区中医药管理局中药壮瑶药医院制剂质量提升项目(GZZJ202026);广西中医药大学校级科研项目(2020MS056)。

摘　　要：目的:建立通脉乐口服液中黄芪、丹参成分的薄层色谱鉴别方法,完善其质量标准。方法:分别以黄芪甲苷、丹酚酸B为对照品,以硅胶G及硅胶GF254为固定相,建立通脉乐口服液中黄芪、丹参的薄层色谱鉴别方法,并对其进行色谱条件耐用性考察。结果:在供试品色谱图中,黄芪、丹参各组分分离良好、斑点对称、清晰圆整,阴性对照无干扰。结论:通脉乐口服液黄芪和丹参成分薄层色谱方法专属性强,操作简便,可用于通脉乐口服液质量控制。Objective:To establish the TLC method for the identification of Astragalus and Salvia miltiorrhiza components in Tongmaile oral liquid and its quality standard was improved.Methods:Using Astragalus A and Salvianolic acid B as reference,silica gel G and silica gel GF254 as stationary phase,TLC identification methods of Astragalus and Salvia miltiorrhiza components in Tongmaile oral liquid were established,and their chromatographic durability was investigated.Results:In the chromatogram of the test product,the TLC spots of Astragalus and Salvia miltiorrhiza components were well separated,the spots were symmetrical,clear,and round,and the negative control had no interference.Conclusion:The TLC methods of Astragalus and Salvia miltiorrhiza components for Tongmaile oral liquid are specific and easy to operate,and can be used for quality control of Tongmaile oral liquid.

关 键 词：通脉乐口服液 黄芪甲苷 丹酚酸B TLC 

分 类 号：R284.2[医药卫生—中药学]