民族药岩黄连生物碱提取与分离工艺研究进展  

Review on Extraction and Separation of Alkaloids from Ethnic Medicine Corydalis Saxicola

作　　者：欧夏莲 蒋莉 李翠[1,2,3,4] 刘寒 唐春风[1,5] 祝梦琦 覃美 黄丽容 张占江[1,2,3,6] Ou Xialian;Jiang Li;Li Cui;Liu Han;Tang Chunfeng;Zhu Mengqi;Qin Mei;Huang Lirong;Zhang Zhanjiang(Guangxi Botanical Garden of Medicinal Plants,Nanning 530023,China;National Center forTCM Inheritance and Innovation,Guangxi Botanical Garden of Medicinal Plant,Nanning 530023,China;Guangxi Key Laboratory of Medicinal Resources Protection and Genetic Improvement,Guangxi Botanical Garden of Medicinal Plants,Nanning 530023,China;Guangxi Engineering Research Center of Traditional Chinese Medicine Resource Intelligent Creation,Nanning 530023,China;National Engineering Research Center for Southwest Endangered Medicinal Materials Resources Development,Guangxi Botanical Garden of Medicinal Plants,Nanning 530023,China;Guangxi Key Laboratory for High-Quality Formation and Utilization of Dao-Di Herbs,Nanning 530023,China)

机构地区：[1]广西壮族自治区药用植物园,广西南宁530023 [2]广西壮族自治区药用植物园国家中医药传承创新中心,广西南宁530023 [3]广西壮族自治区药用植物园广西药用资源保护与遗传改良重点实验室,广西南宁530023 [4]广西壮族自治区药用植物园广西壮族自治区中药资源智慧创制工程研究中心,广西南宁530023 [5]广西壮族自治区药用植物园西南濒危药材资源开发国家工程研究中心,广西南宁530023 [6]广西壮族自治区药用植物园广西道地药材高品质形成与应用重点实验室,广西南宁530023

出　　处：《亚太传统医药》2024年第9期65-72,共8页Asia-Pacific Traditional Medicine

基　　金：广西重点研发计划(桂科AB23026092);广西自然科学基金面上项目(2023GXNSFAA026509,2020GXNSFAA159151);广西中医药管理局广西特色药材关键技术研究推广项目(GZKJ2314);广西中医药适宜技术开发与推广项目(GZSY22-02);广西药用资源保护与遗传改良重点实验室项目(KL2022ZZ04,KL2023ZZ09);广西药用资源保护与遗传改良重点实验室开放课题(KL2023KF01,KL2023KF04)。

摘　　要：岩黄连是我国民族药材之一,广泛应用于医药行业,有较长的使用历史。生物碱是岩黄连主要活性成分,具有清热解毒、消炎止痛、抗病毒、抗氧化、抗肝硬化及纤维化等多种药理活性作用。随着提取、分离技术的飞快发展,开发利用岩黄连的活性成分已成为近年的研究热点。通过梳理文献信息,综述岩黄连中生物碱的提取、分离与鉴定方法。目前,岩黄连生物碱的提取方法可归纳为乙醇提取法、盐酸-甲醇提取法、甲醇提取法和氯仿提取法,其中乙醇提取法、盐酸-甲醇提取法最为常用;分离方法包括色谱法、有机试剂萃取法、沉淀法、结晶法和树脂吸附法;鉴定方法有薄层色谱法及紫外光谱、红外光谱、质谱和核磁共振光谱等波谱法,但目前一些较为先进的提取、分离方法未见应用于岩黄连生物碱的生产。通过综述岩黄连生物碱提取与分离工艺研究进展,旨在为保护、研究与合理开发利用岩黄连药材提供参考依据。As a commonly used ethnic medicine in China,Corydalis Saxicola is widely used in the pharmaceutical industry and has a long history of use.Alkaloids,which are the main active ingredients of Corydalis Saxicola,possess a variety of pharmacological activities such as heat-clearing and detoxifying,anti-inflammatory and analgesic,anti-virus,anti-oxidation,anti-cirrhosis and anti-hepatic fibrosis.With the rapid development of extraction and separation technologies,the utilization of the active components of Corydalis Saxicola has become a research hotspot in recent years.By combing the literature information,the extraction,separation and identification methods of alkaloids in Corydalis Saxicola were reviewed.At present,the extraction methods of alkaloids from Corydalis Saxicola can be summarized as ethanol extraction method,hydrochloric acid-methanol extraction method,methanol extraction method and chloroform extraction method.Among them,ethanol extraction method and hydrochloric acid-methanol extraction method are the most commonly used ones.The separation methods can be classified as chromatography,organic reagent extraction,precipitation,crystallization and resin adsorption.The identification methods include thin layer chromatography,ultraviolet spectroscopy,infrared spectroscopy,mass spectrometry and nuclear magnetic resonance spectroscopy.However,some advanced extraction and separation methods have not been applied to the production of alkaloids from Corydalis Saxicola.This review aims to lay a foundation for the protection,scientific research and utilization of Corydalis Saxicola.

关 键 词：岩黄连 民族药 生物碱 提取分离 

分 类 号：R284.2[医药卫生—中药学]