蜂胶枳椇子复合物对小鼠酒精性肝损伤的保护作用研究  

Study on Protective Effect of Propolis Hovenia Complex on Alcohol Induced Liver Injury in Mice

作　　者：刘丹 张美萍 李英 Liu Dan;Zhang Meiping;Li Ying(Shanxi Zhendong Wuhe Medical Care Hall Co.,Ltd.,Changzhi 047100,China)

机构地区：[1]山西振东五和医养堂股份有限公司,山西长治047100

出　　处：《亚太传统医药》2024年第11期34-37,共4页Asia-Pacific Traditional Medicine

摘　　要：目的:研究蜂胶枳椇子复合物对小鼠乙醇所致急性肝损伤的预防保护作用。方法:实验设5个组,分别为模型对照组、阴性对照组以及高、中、低3个剂量组,各组小鼠12只,3个剂量组每日灌胃受试物的剂量分别为1.8 g/kg、1.2 g/kg、0.6 g/kg,模型对照组和阴性对照组提供等剂量的蒸馏水。灌胃持续30 d后,测定各组小鼠肿瘤坏死因子α(TNF-α)活性、丙二醛(MDA)、三酰甘油(TG)和还原型谷胱甘肽(GSH)含量,并观察肝细胞脂肪变性情况。结果:各剂量组实验前后小鼠体质量对比阴性对照组、模型对照组均未产生显著影响;对比模型对照组,高剂量组的TG含量、MDA含量显著降低,中高剂量组的GSH含量明显升高,且其血清和肝组织匀浆中TNF-α含量显著降低,其病理组织学显示:高剂量组与模型对照组比较,肝细胞脂肪变性程度和肝损伤程度均明显减轻。结论:蜂胶枳椇子复合物可以有效保护和预防酒精诱导下小鼠急性肝损伤。Objective:The preventive and protective effects of propolis Hovenia dulcis complex on acute liver injury induced by alcohol in mice was evaluated in this paper.Methods:Five groups were set up in the experiment,which were model group,negative group,and high,medium,and low dose groups,with 12 mice in each group.The daily dose of test objects in the three dose groups was 1.8 g/kg,1.2 g/kg,and 0.6 g/kg,respectively.The model group and the negative group were provided with the same amount of distilled water.After 30 days of continuous intragastric administration,tumor necrosis factor(TNF-α)activity,malondialdehyde(MDA),triglyceride(TG),and reduced glutathione(GSH)contents were measured,and the steatosis of hepatocytes was observed.Results:There was no significant effect on the weight of mice in the negative group and the model group before and after the experiment.Compared with model group,TG content and MDA content were significantly decreased in the high-dose group,GSH content was significantly increased in the medium-high dose group,and TNF-αcontent in serum and liver homogenate was decreased considerably.The histopathology showed that compared with the model control group,the degree of hepatocyte steatosis and liver injury were significantly reduced in the high-dose group.Conclusion:Propolis Hovenia compound can effectively protect and prevent acute liver injury induced by alcohol in mice.

关 键 词：蜂胶 枳椇子 葛根 酒精性肝损伤 

分 类 号：R285.5[医药卫生—中药学]