山东省聊城市部分地区猪场PRRS病原检测与基因序列分析  

Pathogen Detection and Genetic Sequence Analysis of PRRS on Swine Farms in Liaocheng City,Shandong Province

作　　者：刘来可 刘东莲 Liu Laike;Liu Donglian(Yanggu Animal Husbandry and Veterinary Development Center,Yanggu 252300,Shandong,China)

机构地区：[1]阳谷县畜牧兽医事业发展中心,山东阳谷252300

出　　处：《中国动物检疫》2024年第11期28-33,共6页China Animal Health Inspection

摘　　要：为了解聊城市猪繁殖与呼吸综合征病毒(PRRSV)的感染与遗传变异情况,2021年10月—2023年12月从4个县区的52个猪场采集疑似感染PRRSV病猪的血清、组织和精液样品等共588份,采用RT-qPCR法进行PRRSV核酸检测,采用RT-PCR法扩增部分PRRSV核酸阳性样品的ORF5基因序列,分析其基因型和遗传变异情况。结果显示:23个猪场中的101份样品检测为PRRSV核酸阳性,场阳性检出率和样品阳性检出率分别为44.23%(23/52)和17.18%(101/588);不同地区和不同季节来源样品PRRSV核酸阳性检出率分别为12.38%~20.45%和12.64%~20.33%;高致病型、经典型、类NADC30型和类NADC34型占比分别为10.89%、28.71%、54.46%和5.94%。共扩增获得9份PRRSV ORF5基因序列,其中5份与类NADC30株、3份与高致病性株、1份与经典株相似性最高;不同基因型样品的GP5蛋白均出现氨基酸位点变化,其中类NADC30型突变率最高。结果说明:聊城市4个地区存在PRRSV感染,且一年四季皆可发生;流行病原基因型复杂,其中类NADC30和高致病型为主要流行基因型,同时存在病原变异风险。建议持续监测猪场PRRSV流行情况与基因型特征,分析毒株基因序列变异情况,避免PRRSV流行对该地区猪场造成的损失。In order to investigate the status of infection and genetic variation of porcine reproductive and respiratory syndrome virus(PRRSV)in Liaocheng City,588 samples of serum,tissue and semen from sick pigs suspected of PRRSV were collected from 52 farms in 4 counties/cities from October to December 2023 to detect nucleic acids of PRRSV by RT-qPCR.ORF5 gene sequences of some positive PRRSV samples were amplified by RT-PCR to analyze their genotypes and genetic variation.The results showed that 101 positive samples were detected from 23 farms,and the positive detection rates of farms and samples were 44.23%(23/52)and 17.18%(101/588),respectively:the detection rates of positive PRRSV nucleic acids of samples from different regions and in different seasons were 12.38%-20.45% and 2.64%-20.33%,respectively;high pathogenic,classical,NADC30-like and NADC34-like strains were 10.89%,28.71%,54.46% and 5.94%,respectively.9 PRRSV ORF5 gene sequences were obtained through amplification,including 5 highest similar to those of NADC30-like stains,3 to those of pathogenic strains and 1 to that of classical strain;amino acid site mutation was observed in GP5 proteins of the samples under different genotypes,especially NADC30-like strains with highest mutation rate.In conclusion,PRRSV infection was available in 4 regions of Liaocheng,likely all year round;for the complex epidemiological genotypes,NADC30-like and highly pathogenic strains were dominant,with a risk of pathogen mutation.Thus,it was recommended to continuously monitor the prevalence and genotypic characteristics of PRRSV on swine farms,analyze the genetic sequence variation of virulent strains,so as to avoid potential loss that might be caused by PRRSV to local farms.

关 键 词：猪繁殖与呼吸综合征病毒 分子流行病学 ORF5基因 遗传变异分析 

分 类 号：S851.3[农业科学—预防兽医学]