紫草素调节Hippo信号通路对人增生性瘢痕成纤维细胞增殖和凋亡的影响  

Effect of shikonin on the proliferation and apoptosis of human hypertrophic scar fibroblasts by regulating the Hippo signaling pathway

作　　者：高虎 张祥明[1] 曾静[1] GAO Hu;ZHANG Xiangming;ZENG Jing(Wound Repair&Rehabilitation Center,Wuhan Third Hospital&Tongren Hospital of Wuhan University,Wuhan 430074,Hubei,China)

机构地区：[1]武汉市第三医院/武汉大学附属同仁医院光谷院区皮肤创面修复中心,湖北武汉430074

出　　处：《武汉大学学报（医学版）》2024年第11期1285-1290,共6页Medical Journal of Wuhan University

基　　金：武汉市卫生健康委员会资助项目(编号:WZ22C15)。

摘　　要：目的探究紫草素对人增生性瘢痕成纤维细胞(HSF)行为的影响及相关分子机制。方法将HSF细胞分为对照组、紫草素低剂量组、紫草素中剂量组、紫草素高剂量组、XMU-MP-1(Hippo信号通路抑制剂)组。CCK-8检测细胞增殖;Transwell实验分别检测各组细胞迁移和侵袭能力;采用流式细胞术检测细胞凋亡;Western Blot检测Col-Ⅰ、Col-Ⅲ、α-SMA、Bcl-2、Bax、YAP、TAZ蛋白。结果与对照组相比,紫草素低、中、高剂量组HSF细胞增殖率、细胞迁移和侵袭个数、Col-Ⅰ、Col-Ⅲ、α-SMA、Bcl-2、YAP、TAZ蛋白表达显著降低,细胞凋亡率、Bax蛋白表达显著升高(P<0.05);XMU-MP-1组与紫草素高剂量组HSF细胞以上各项检测指标均无统计学差异(P>0.05)。结论紫草素通过抑制Hippo信号通路可抑制人增生性瘢痕成纤维细胞增殖和迁移,促进其凋亡。Objective To investigate the effect of shikonin on the behavior of human hypertrophic scar fibroblasts(HSF)and its related molecular mechanisms.Methods HSF cells were divided into control group,low dose shikonin group,medium dose shikonin group,high dose shikonin group,and XMU-MP-1(Hippo signaling pathway inhibitor)group;CCK-8 was applied to detect cell proliferation;Transwell experiment was applied to detect the migration and invasion abilities of cells in each group;flow cytometry was applied to detect cell apoptosis;Western Blot was applied to detect Col-Ⅰ,Col-Ⅲ,α-SMA,Bcl-2,Bax,YAP,and TAZ proteins.Results Compared with those in the control group,the cell proliferation rate,numbers of cell migration and invasion,and expression of ColⅠ,ColⅢ,α-SMA,Bcl-2,YAP,and TAZ proteins in the low,medium,and high dose shikonin groups were significantly decreased,the apoptosis rate and expression of Bax protein were significantly increased(P<0.05);there was no statistically significant difference in the above detection indicators of HSF cells between the XMU-MP-1 group and the high-dose shikonin group(P>0.05).Conclusion Shikonin can inhibit the proliferation and migration of human hypertrophic scar fibroblasts and promote their apoptosis by inhibiting the Hippo signaling pathway.

关 键 词：紫草素 Hippo信号通路 人增生性瘢痕成纤维细胞 增殖 凋亡 

分 类 号：R622[医药卫生—整形外科]