HIF-1α基因rs11549465多态性与青海世居藏族胃癌患者幽门螺杆菌易感性的相关性研究  

Correlation research of the polymorphism of HIF-1a gene rs11549465 and susceptibility to Helicobacter pylori in Tibetan gastric cancer patients living in Qinghai province

作　　者：李苏华 李焱 王雅婷 张孟君 安琪 王学红 LI Su-hua;LI Yan;WANG Ya-ting;ZHANG Meng-jun;AN Qi;WANG Xue-hong(Department of Gastroenterology,Affiliated Hospital of Qinghai University,Xining 810001,Qinghai Province,China)

机构地区：[1]青海大学附属医院消化内科,青海西宁810001

出　　处：《中国临床药理学杂志》2024年第22期3239-3243,共5页The Chinese Journal of Clinical Pharmacology

基　　金：青海省自然科学基金基础研究计划(青年)基金资助项目(2022-ZJ-969Q);青海省“昆仑英才·高端创新创业人才”基金资助项目(2020-18)。

摘　　要：目的观察缺氧诱导因子1α(HIF-1α)基因rs11549465多态性与青海世居藏族胃癌幽门螺杆菌(Hp)易感性的关系。方法将青海世居藏族胃癌患者按队列法分为Hp感染组和非Hp感染组。用聚合酶链反应法检测静脉血中HIF-1α基因rs11549465多态性,以及癌组织中HIF-1α、血管内皮生长因子(VEGF)、P53、Maspin的表达水平;用免疫组化法检测癌组织中HIF-1α、VEGF、P53、Maspin的阳性表达情况。结果Hp感染组入组48例,非Hp感染组入组31例。青海世居藏族胃癌Hp感染组和非Hp感染组HIF-1α基因rs11549465位点上CC基因频率分别为58.33%(28例/48例)和83.87%(26例/31例),在统计学上差异有统计学意义(P<0.05)。Logistic回归分析显示:HIF-1α基因rs11549465位点上CT基因型是青海世居藏族胃癌患者Hp感染的影响因素(比值比=5.026,95%置信区间=1.810~13.951,P<0.05)。Hp感染组和非Hp感染组癌组织中HIF-1α阳性表达率分别为89.58%和64.52%,VEGF阳性表达率分别为91.67%和70.97%,P53阳性表达率分别为31.25%和54.84%,Maspin阳性表达率分别为29.17%和51.61%,HIF-1αmRNA相对表达水平分别为2.48±0.41和2.23±0.39,VEGF mRNA相对表达水平分别为2.42±0.38和2.14±0.31,P53 mRNA相对表达水平分别为1.99±0.33和2.27±0.35,Maspin mRNA相对表达水平分别为2.06±0.32和2.31±0.36,在统计学上差异均有统计学意义(均P<0.05)。结论HIF-1α基因rs11549465位点CT基因型是青海世居藏族胃癌患者Hp易感性的高危因素,且HIF-1α可以通过调控VEGF、P53、Maspin信号转导通路表达来发挥防治胃癌的作用。Objective To observe the relationship between the rs11549465 polymorphism of hypoxia-inducible factor 1α(HIF-1α)gene and susceptibility to Helicobacter pylori(Hp)infection in the Tibetan gastric cancer patients residing in Qinghai province.Methods The Tibetan gastric cancer patients residing in Qinghai province were divided into Hp infection and non-HP infection groups by cohort method.The rsl1549465 polymorphism of the HIF-1αgene in venous blood,as well as the expression levels of HIF-1α,vascular endothelial growth factor(VEGF),P53 and Maspin in cancer tissue,were measured by polymerase chain reaction.Immunohistochemistry was used to detect the positive expressions of HIF-1α,VECF,P53 and Maspin in cancer tissues.Results Hp infection and non-HP infection groups were enrolled 48 cases and 31 cases,respectively.The CC gene frequency at the HIF-1lαrsl1549465 sites in the Hp infection and non-HP infection groups were 58.33%(28 cases/48 cases)and 83.87%(26 cases/31 cases),with a statistically significant difference(P<0.05).Logistic regression analysis showed that CT genotype at HIF-lαrs11549465 locus was the influencing factor of Hp infection in the the Tibetan gastric cancer patients residing in Qinghai province(odds ratio=5.026,95%confidence interval=1.810-13.951,P<0.05).The positive expression rates of HIF-lαin Hp infection and non-HP infection groups were 89.58%and 64.52%;positive expression rates of VEGF were 91.67%and 70.97%;positive expression rates of P53 were 31.25%and 54.84%;positive expression rates of Maspin were 29.17%and 51.61%;the relative expression levels of HIF-1αmRNA were 2.48±0.41 and 2.23±0.39;the relative expression levels of VEGF mRNA were 2.42±0.38 and 2.14±0.31;the relative expression levels of P53 mRNA were 1.99±0.33 and 2.27±0.35;the relative expression levels of Maspin mRNA were 2.06±0.32 and 2.31±0.36,the differences were statistically significant(all P<0.05).Conclusion The CT genotype at HIF-1αrs11549465 locus is a high-risk factor for Hp susceptibility the Tibetan gastr

关 键 词：缺氧诱导因子1Α rs11549465基因多态性 藏族 胃癌 

分 类 号：R97[医药卫生—药品]