金水六君煎对哮喘小鼠气道炎症及IFN-γ/IL-4失衡的影响研究  

Effects of Jinshui Liujun decoction on airway inflammation and IFN-γ/IL-4 imbalance in asthmatic

作　　者：王志旺 席建宏[1] 梁可克 黄柯婷 赵跃 杨瑛 程小丽 段海婧 任远 WANG Zhi-wang;XI Jian-hong;LIANG Ke-ke;HUANG Ke-ting;ZHAO Yue;YANG Ying;CHENG Xiao-li;DUAN Hai-jing;REN Yuan(College Pharmacy,Gansu University of Chinese Medicine,Lanzhou 730000,Gansu Province,China;Gansu Pharmaceutical Industry Innovation Research Institute,Gansu University of Chinese Medicine,Lanzhou 730000,Gansu Province,China;Teaching Experiment and Training Center,Gansu University of Chinese Medicine,Lanzhou 730000,Gansu Province,China;Pharmacy Department,The Third People's Hospital of Gansu Province,Lanzhou 730020,Gansu Province,China)

机构地区：[1]甘肃中医药大学药学院,甘肃兰州730000 [2]甘肃中医药大学陇药产业创新研究院,甘肃兰州730000 [3]甘肃中医药大学教学实验实训中心,甘肃兰州730000 [4]甘肃省第三人民医院药剂科,甘肃兰州730020

出　　处：《中国临床药理学杂志》2024年第22期3280-3284,共5页The Chinese Journal of Clinical Pharmacology

基　　金：国家自然科学基金资助项目(82260852、81460668);甘肃省自然科学基金资助项目(20JR5RA183、1606RJZA011,1310RJZA086)。

摘　　要：目的研究金水六君煎对哮喘小鼠气道炎症及干扰素-γ(IFN-γ)/白细胞介素-4(IL-4)失衡的影响。方法用注射、雾化吸入卵清白蛋白(OVA)以复制哮喘模型。将小鼠分为模型组、阳性药对照组(地塞米松1.0mg·kg^(-1))以及低、中、高剂量实验组(分别灌胃给予4.1、8.2、16.4g·kg^(-1)金水六君煎),空白组与模型组灌胃纯净水,连续干预2周。用肺功能仪测定小鼠肺功能指标【潮气量(TV)、呼吸频率(RR)】,用酶联免疫吸附试验(ELISA)法测定支气管肺泡灌洗液(BALF)中IFN-γ、IL-4水平,用荧光定量聚合酶链反应法测定肺组织IFN-γ、IL-4mRNA相对表达水平。结果空白组、模型组、阳性药对照组和低、中、高剂量实验组的TV分别为(118.90±24.20)、(87.10±13.36)、(112.70±18.92)、(105.20±15.24)、(107.60±16.47)和(110.10±17.41)μL,RR分别为(126.60±17.57)、(178.80±31.06)、(133.00±19.45)、(146.00±25.82)、(141.30±24.78)和(137.00±22.81)bpm,IFN-γ水平分别为(110.95±9.26)、(60.16±2.75)、(98.09±8.21)、(79.58±6.49)、(88.16±7.58)和(97.26±7.23)pg·mL^(-1) IL-4水平分别为(118.06±8.47)、(416.08±22.21)、(272.17±13.14)、(319.34±15.27)、(298.77±14.83)和(278.71±14.24)pg·mL^(-1),肺组织IFN-γmRNA相对表达水平分别为2.70±0.08、1.00±0.00、2.65±0.06、1.89±0.04、2.24±0.05和2.58±0.06,IL-4mRNA相对表达水平分别为0.25±0.02、1.00±0.00、0.26±0.02、0.61±0.05、0.50±0.03和0.33±0.03。模型组的上述指标与空白组比较,低、中、高剂量实验组的上述指标与模型组比较,在统计学上差异均有统计学意义(P<0.05,P<0.01)。结论金水六君煎具有一定的平喘作用,缓解IFN-γ/IL-4免疫失衡而抑制气道炎症是其作用机制之一。Objective To study the effects of Jinshui Liujun decoction(JLD)on airway inflammation and interferon-γ(IFN-γ)/interleukin-4(IL-4)imbalance in asthma.Methods The ovalbumin(OVA)was used to replicate the asthmatic mouse by injection and nebulization inhalation.Asthmatic mice were randomly divided into model group,positive control group(1.0 mg·kg^(-1)dexamethasone)and experimental-L,-M,-H groups(4.1,8.2 and 16.4 g·kg^(-1)JLD).The blank group and the model group were given purified water by gavage for 2 consecutive weeks.The lung function[tidal volume(TV)and respiratory rate(RR)]of mice was measured by lung function test system after administering JLD by gavage.IFN-γ,IL-4 in bronchoalveolar lavage fluid(BALF)and their genes in lung tissue were determined through enzyme-linked immunosorbent assay and quantitative real-time polymerase chain reaction respectively.Results The TV levels in blank group,model group,positive control group and experimental-L,-M,-H groupswere(118.90±24.20),(87.10±13.36),(112.70±18.92),(105.20±15.24),(107.60±16.47)and(110.10±17.41)μL;RRlevelswere(126.60±17.57),(178.80±31.06),(133.00±19.45),(146.00±25.82),(141.30±24.78)and(137.00±22.81)bpm;IFN-γlevels were(110.95±9.26),(60.16±2.75),(98.09±8.21)、(79.58±6.49)、(88.16±7.58)and(97.26±7.23)pg·mL^(-1);IL4 levels were(118.06±8.47)、(416.08±22.21)、(272.17±13.14)、(319.34±15.27)、(298.77±14.83)and(278.71±14.24)pg·mL-1;the IFN-γmRNA levels were 2.70±0.08,1.00±0.00,2.65±0.06,1.89±0.04,2.24±0.05 and 2.58±0.06;IL-4 mRNA levels were 0.25±0.02,1.00±0.00,0.26±0.02,0.61±0.05,0.50±0.03 and 0.33±0.03,respectively.There were statistically significant differences between the above indicators of model group and blank group,and experimental-L,-M,-H groups and model group(P<0.05,P<0.01).Conclusion JLD has a certain antiasthmatic effect and one of mechanisms is to alleviate the IFN-γ/IL-4 immune imbalance and inhibit airway inflammation.

关 键 词：金水六君煎 哮喘 气道炎症 干扰素-γ/白细胞介素-4失衡 

分 类 号：R28[医药卫生—中药学]