山楂叶总黄酮对糖尿病大鼠周围神经病变影响的研究  

Experimental Study of Hawthorn Leaves Flavonoids on Peripheral Neuropathy of Diabetic Rats

作　　者：陈熠飞 黄广为 陶琳[1] 马婷[1] 齐淑芳[3] CHEN Yifei;HUANG Guangwei;TAO Lin;MA Ting;QI Shufang(The First Department of Neurology,Jiamusi Central Hospital,Jiamusi 154002,Heilongjiang,China;The Second Department of Neurology,Jiamusi Central Hospital,Jiamusi 154002,Heilongjiang,China;School of Basic Medical Sciences,Jiamusi University,Jiamusi 154007,Heilongjiang,China)

机构地区：[1]佳木斯市中心医院神经内一科,黑龙江佳木斯154002 [2]佳木斯市中心医院神经内二科,黑龙江佳木斯154002 [3]佳木斯大学基础医学院,黑龙江佳木斯154007

出　　处：《中国药物滥用防治杂志》2024年第11期2022-2026,共5页Chinese Journal of Drug Abuse Prevention and Treatment

基　　金：黑龙江省卫生健康委科研课题(编号:20210303070182)。

摘　　要：目的:探讨山楂叶总黄酮(HLF)对糖尿病大鼠周围神经病变(DPN)的影响。方法:以腹腔注射STZ致糖尿病大鼠为研究对象,随机分为正常组、模型组,HLF低、中、高剂量组和弥可保组。给药12周后,检测大鼠的热痛阈值和坐骨神经传导速度,观察坐骨神经和胰腺组织结构变化,同时进行体重、空腹血糖、血清丙二醇(MDA)及血清谷胱甘肽(GSH)检测。结果:给药12周后,各组体重、血糖、热痛阈值、神经传导速度、MDA及GSH水平均与正常组有显著差异(P<0.05)。HLF各组体重重于模型组(P<0.05),HLF高、中组体重重于弥可保组(P<0.01);HLF各组血糖低于模型组与弥可保组(P<0.01);HLF各组及弥可保组热痛阈值低于模型组(P<0.01),HLF低组热痛阈值低于弥可保组(P<0.01);HLF各组及弥可保组MNCV、SNCV高于模型组(P<0.05),HLF低组MNCV低于弥可保组(P<0.01),HLF中、低组SNCV低于弥可保组(P<0.05);HLF各组MDA低于模型组与弥可保组(P<0.05),HLF高、中组GSH高于模型组与弥可保组(P<0.01)。光镜下可见模型组、弥可保组坐骨神经及胰腺组织结构损伤性明显,HLF各组损伤性减轻。结论:HLF可降低血糖,缓解痛阈值升高,减低坐骨神经传导速度,改善坐骨神经及胰腺组织结构损伤,延缓DPN的发生及发展。Objective:To investigate the effect of hawthorn leaves flavonoids(HLF)on peripheral neuropathy(DPN)in diabetes rats.Methods:Diabetes rats induced by intraperitoneal injection of STZ were randomly divided into normal group,model group,low,medium and high dose HLF groups and mecobalamin group.After 12 weeks of administration,the thermal pain threshold and sciatic nerve conduction velocity of rats were measured,and changes in sciatic nerve and pancreatic tissue structure were observed.At the same time,body weight,fasting blood glucose,serum propylene glycol(MDA),and serum glutathione(GSH)were measured.Results:After 12 weeks of administration,the body weight,blood glucose,heat pain threshold,nerve conduction velocity,MDA and GSH levels in each group were significantly different from those in the normal group(P<0.05).The body weight of each HLF group was heavier than that of the model group(P<0.05),and the body weight of the HLF high and medium groups was heavier than that of the mecobalamin group(P<0.01).The blood glucose in each HLF group was lower than that in the model group and the mecobalamin group(P<0.01).The thermal pain threshold of HLF groups and mecobalamin group was lower than that of model group(P<0.01),and the thermal pain threshold of HLF low group was lower than that of mecobalamin group(P<0.01).MNCV and SNCV in HLF groups and mecobalamin group were higher than those in model group(P<0.05).MNCV in HLF low group was lower than that in mecobalamin group(P<0.01).SNCV in HLF medium and low groups was lower than that in mecobalamin group(P<0.05).MDA in HLF groups was lower than that in model group and mecobalamin group(P<0.05).GSH in HLF high and medium groups was higher than that in model group and mecobalamin group(P<0.01).Under the light microscope,the damage of sciatic nerve and pancreatic tissue structure in the model group and the mecobalamin group was obvious,and the damage in the HLF group was reduced.Conclusion:HLF can lower blood sugar,alleviate elevated pain threshold,reduce sciatic nerve conduct

关 键 词：山楂叶总黄酮 糖尿病周围神经病变 热痛阈值 坐骨神经传导速度 

分 类 号：R285.5[医药卫生—中药学]