荆州市三株猫泛白细胞减少症病毒的分离鉴定及其进化分析  

Isolation,identification and evolutionary analysis of three feline panleukopenia virus strains in Jingzhou city

作　　者：吕熙 王运航 邱实 郭慧琛[2] 孙世琪[2] 杨丰利[1] 白满元 LÜXi;WANG Yunhang;QIU Shi;GUO Huichen;SUN Shiqi;YANG Fengli;BAI Manyuan(College of Animal Science and Technology,Yangtze University,Jingzhou 434000,China;State Key Laboratory for Animal Disease Control and Prevention/College of Veterinary Medicine of Lanzhou University/Lanzhou Veterinary Research Institute,Chinese Academy of A gricultural Sciences,Lanzhou 730046,China)

机构地区：[1]长江大学动物科学技术学院,湖北荆州434000 [2]中国农业科学院兰州兽医研究所兰州大学动物医学与生物安全学院动物疫病防控全国重点实验室,甘肃兰州730046

出　　处：《中国兽医科学》2024年第11期1502-1509,共8页Chinese Veterinary Science

基　　金：兰州市人才创业创新项目(2023-RC-3);甘肃省自然科学基金项目(23JRRA554);博士后科学基金项目(2023M733819);甘肃省科技重大专项(23ZDNA007);国家重点研发计划项目(2021YFD1800303,2022YFD1800603)。

摘　　要：为了解我国湖北省荆州市猫泛白细胞减少症病毒(feline panleukopenia virus,FPV)的流行状况、遗传变异和演化方向,本研究采集荆州市4例疑似FPV病死猫肠道样本、1例FPV阳性粪便样本。经PCR检测后将FPV阳性样品接种F81细胞,连续盲传至出现显著细胞病变(cytopathic effect,CPE),利用PCR、间接免疫荧光实验(IFA)、电子显微镜等技术对其进行鉴定。经鉴定,共分离出3株FPV,分别命名为FPV-JZ202201、FPV-JZ202202和FPV-JZ202203。然后进行VP2基因测序并构建进化树。结果显示,此3株FPV的VP2序列与国内外参考毒株的核苷酸同源性为97.9%~99.9%,氨基酸序列同源性为97.6%~99.9%;其中FPV-JZ202203与2023年河北省保定市分离毒株OR727316处于同一分支,与国内疫苗株的距离较远,处于不同的拓朴亚群,FPV-JZ202201和FPV-JZ202202与国内当前流行的其他FPV毒株以及现有的疫苗株在遗传上表现出较高的相似性,且它们均归属于同一遗传拓扑群。本研究结果对于了解FPV的流行病学特征具有重要意义。它不仅增加了我们对FPV流行毒株遗传背景的认识,还为未来猫泛白细胞减少症(FPL)疫苗的研发和优化提供了宝贵的基础数据。In order to understand the prevalence,genetic variation and evolution direction of feline panleukopenia virus in Jingzhou city,Hubei province,China.In this study,four intestinal samples of suspected FPV dead cats and one fecal sample of FPV positive were collected from Jingzhou city.After PCR detection,FPV-positive samples were inoculated into F81 cells and continuously passed blindly until significant cellular lesions appeared.They were identified using techniques such as PCR,indirect immunofluorescence experiments,and electron microscopy.After identification,a total of 3 FPV strains were isolated and named FPV-JZ202201,FPV-JZ202202 and FPV-JZ202203,respectively.The VP2 gene was sequenced and an evolutionary tree was constructed.The results showed that the VP2 sequences of these three FPV strains had 97.9%to 99.9%nucleotide homology and 97.6%to 99.9%amino acid sequence homology with domestic and foreign reference strains,respectively.Among them,FPV-JZ202203 is in the same branch as the OR727316 isolate from Baoding city,Hebei province in 2023,which is far away from the domestic vaccine strain and is in a different topological subgroup.FPV-JZ202201 and FPV-JZ202202share the same characteristics with the domestic epidemic strain and vaccine strain,and they belong to the same genetic topological group.This study enrich the epidemiological data and circulating strain information of FPV in China,and provide reference materials for further development of FP vaccines.

关 键 词：猫泛白细胞减少症病毒 VP2 分离鉴定 序列分析 

分 类 号：S852.659.2[农业科学—基础兽医学]