农田黑土不同提取样本细菌群落结构与功能的对比分析  

Comparative Analysis of Bacterial Community Structure and Func⁃tion in Different Samples of Farmland Black Soil

作　　者：宋时雨 赵丹琪 顾正毅 崔俊涛[1] SONG Shiyu;ZHAO Danqi;GU Zhengyi;CUI Juntao(College of Resources and Environment,Jilin Agricultural University,Changchun 130118,China)

机构地区：[1]吉林农业大学资源与环境学院,长春130118

出　　处：《吉林农业大学学报》2024年第5期836-843,共8页Journal of Jilin Agricultural University

基　　金：吉林省科技厅重点研发项目(20210204170YY)。

摘　　要：以黑土为供试土壤,通过16SrDNA技术,针对从风干复湿土壤离心所得底泥和上清液中获得的DNA与直接提取的原土样本DNA进行测序,探究不同样本在微生物群落多样性、群落结构和代谢途径方面的异同。结果表明:底泥样本OTU数目与原土更为接近,上清液中微生物多样性最低,3种处理之间原核微生物群落组成无显著差异(P>0.05),各处理间优势菌门一致,仅相对丰度存在差异,样本间代谢途径几乎完全一致。所得的底泥与上清液中提取的细胞能够较好地反映土壤中的主要微生物群落结构,未来可继续此方法的扩展研究。探索高质量提取土壤微生物细胞的技术方法,以适应不断发展的现代分子生物学技术。Taking black soil as the test soil,16SrDNA technology was used to sequence the DNA obtained from the sediment and supernatant centrifuged from air dried and rehumidified soil and the DNA extracted from the original soil samples directly to observe the similarities and differences in microbial community diversity,community structure and metabolic pathways.The number of OTU in sediment samples was closer to that in the original soil,and the microbial diversity in the supernatant was the lowest.Meanwhile,there was no significant difference in the composition of prokaryotic microbial community among the three treatments(P>0.05).The dominant bacterial phyla were consistent among all treatmensts,with only differences in relative abundance,and,the metabolic pathways between samples were almost identical.The cells extracted from the sediment and supernatant obtained can better reflect the main microbial community structure in the soil.It can been seen that the extended research of this method can be continued in the future.The study was conducted to explore the technical methods of extracting soil microbial cells with high quality to adapt to the continuous development of modern molecular biology technology to sequence the DNA obtained from the sedi ment and supernatant centrifuged from air dried and rehumidified soil,as well as the DNA directly extracted from the original soil samples,to explore the similarities and differences in microbial community diversity,community structure,and metabolic pathways among different samples.

关 键 词：黑土 底泥 原核微生物 细菌群落结构 微生物组成 

分 类 号：S154.3[农业科学—土壤学]