金骨莲胶囊及其拆方抗炎镇痛作用的研究  

Study on anti-inflammatory and analgesic effects of Jingulian capsules and its disassembled formulas

作　　者：王玲 鲁婷婷 周祖英 王华斌 朱迪 肖金超 李勇军[1,5] 郑林[1,5] 黄勇[1,5] WANG Ling;LU Tingting;ZHOU Zuying;WANG Huabin;ZHU Di;XIAO Jinchao;LI Yongjun;ZHENG Lin;HUANG Yong(Guizhou Provincial Key Laboratory of Pharmaceutics/State Key Laboratory of Functions and Applications of Medicinal Plants,Guiyang 550004,Guizhou,China;School of Pharmaceutical Sciences,Guizhou Medical University,Guiyang 550004,Guizhou,China;Guizhou University of Traditional Chinese Medicine,Guiyang 550004,Guizhou,China;Drug Preparation Center of the First Affiliated Hospital of Guizhou University of Traditional Chinese Medicine,Guiyang 550004,Guizhou,China;National Miao Medicine Engineering Technology Research Center,Guizhou Medical University,Guiyang 550004,Guizhou,China)

机构地区：[1]贵州医科大学贵州省药物制剂重点实验室&省部共建药用植物功效与利用国家重点实验室,贵州贵阳550004 [2]贵州医科大学药学院,贵州贵阳550004 [3]贵州中医药大学,贵州贵阳550004 [4]贵州中医药大学第一附属医院制剂中心,贵州贵阳550004 [5]贵州医科大学国家苗药工程技术研究中心,贵州贵阳550004

出　　处：《贵州医科大学学报》2024年第11期1609-1614,共6页Journal of Guizhou Medical University

基　　金：国家自然科学基金(81960763);贵州省科技计划项目(黔科合平台人才GCC[2022]031-1,黔科合平台人才GCC[2019]5407,黔科合平台人才GCC[2023]006);贵阳市科技计划项目(筑科合同[2019]9-2-33);贵州中医药大学2021年度科研创新和探索专项项目(2018YFC170810203)。

摘　　要：目的研究金骨莲胶囊的抗炎镇痛作用及各组方配伍的抗炎镇痛内在规律。方法108只健康昆明种小鼠随机分为对照组(CON组,0.5%羧甲基纤维素钠)、阳性药组(ASPL组,阿司匹林)、全方组(QF组,金骨莲胶囊)、主药组(A组,金铁锁)、辅药组(B组,透骨香、汉桃叶、八角枫)、引药组(C组,大血藤)、主+辅组(A+B组)、主+引组(A+C组)及辅+引组(B+C组)共9组,采用小鼠耳廓肿胀实验评价金骨莲胶囊的抗炎作用,采用醋酸致小鼠扭体反应实验评价其镇痛作用,并结合正交t值法探讨金骨莲方的组方配伍内在规律。结果与CON相比,ASPL组、QF组、A组、B组、C组、A+B、A+C组以及B+C组的小鼠耳廓肿胀抑制率和扭体抑制率均有不同程度的升高(P<0.05或P<0.01),且QF组耳廓肿胀抑制率和扭体抑制率最高;正交t值表显示,QF组能显著抑制小鼠耳廓肿胀度(P<0.01)和小鼠扭体次数(P<0.05),交互作用明显。结论金骨莲胶囊具有显著的抗炎镇痛作用,拆方研究表明该方存在配伍增强效应。Objective To explore the anti-inflammatory and analgesic effects of Jingulian capsules and the inherent laws of anti-inflammatory and analgesic effects of formula compatibility.Methods A total of 108 healthy Kunming mice were randomly divided into 9 groups:control group(CON group,0.5%Carboxymethylcellulose sodium),positive drug group(ASPL group,aspirin),whole prescription group(QF group,Jingulian Capsules),main drug group(A group,Psammosilene tunicoides),auxiliary drug group(B group,Gaultheriae Yunnanensis Herba seu Radix,Schefflerae Leucanthae Ramulus et Folium and Alangii Radix),lead drug group(C group,Sargentodoxae Caulis),main+auxiliary group(A+B group),main+lead group(A+C group),and auxiliary+lead group(B+C group).Mouse ear swelling experiment was performed to evaluate anti-inflammatory effect of Jingulian Capsule.Analgesic effect of Jingulian was evaluated by acetic acid-induced writhing body reaction experiment in mice.The inherent compatibility law among each formula in Jingulian prescription were discussed by orthogonal t-value method.Results When compared to CON group,the inhibition rates of mouse ear swelling and torsion were both increased to varying degrees in ASPL,QF,A,B,C,A+B,A+C,and B+C groups(P<0.05 or P<0.01).QF group had the highest inhibition rates for ear swelling and torsion.The orthogonal t-value table showed that QF group could significantly inhibit the degree of mouse ear swelling(P<0.01)and the number of mouse twisting movements(P<0.05)with a significant interaction effect.Conclusion Jingulian capsule has significant anti-inflammatory and analgesic effects,and disassembled formulas show that the formulas have a compatibility enhancement effect.

关 键 词：金骨莲胶囊 抗炎 镇痛 正交T值法 拆方 

分 类 号：R961[医药卫生—药理学]