基于图像识别技术发掘水稻耐旱性QTL  

Drought Tolerance QTLs were Explored Based on Image Recognition Technology in Rice

作　　者：李恩熙 冯芳君[2] 马超[3] 胡冬 田明璐 班松涛 李琳一[3] 刘鸿艳[2] 吴文嫱[1] 马孝松[2] Li Enxi;Feng Fangjun;Ma Chao;Hu Dong;Tian Minglu;Ban Songtao;Li Linyi;Liu Hongyan;Wu Wengqiang;Ma Xiaosong(School of Life Sciences,Hainan University,Haikou,570228;Key Laboratory of Grain Crop Genetic Resources Evaluation and Utilization(Ministry of Agriculture and Rural Affairs),Shanghai Agrobiological Gene Center,Shanghai,201106;Institute of Agricultural Science and Technology Information,Shanghai Academy of Agricultural Sciences,Shanghai,201403)

机构地区：[1]海南大学生命科学学院,海口570228 [2]上海市农业生物基因中心,农业农村部粮食作物基因资源评价利用重点实验室,上海201106 [3]上海市农业科学院,农业科技信息研究所,上海201403

出　　处：《分子植物育种》2024年第23期7728-7736,共9页Molecular Plant Breeding

基　　金：上海市自然科学基金项目(19ZR1446700)资助。

摘　　要：为了快速鉴定水稻耐旱性基因资源和耐旱候选基因,本研究建立了一种基于图像识别技术测量水稻在PEG-6000(聚乙二醇)模拟干旱胁迫和H2O2胁迫处理条件下的绿叶率反映水稻耐旱性的方法,相关性分析表明图像识别技术测量的绿叶率与实测的绿叶比重呈极显著正相关,相关系数大于0.85。利用该方法测量了‘珍汕97B’‘/IRAT109’重组自交系群体在PEG-6000(聚乙二醇)模拟干旱胁迫和H2O2胁迫处理条件下的绿叶率,并结合该群体的基因型数据开展了QTL定位分析,两种胁迫处理条件下分别定位了4个QTL。这些定位的QTL将有助于理解水稻耐旱性的遗传基础,同时本研究鉴定获得的耐旱材料和定位的耐旱QTL将为水稻耐旱育种提供基因资源。To quickly identify drought tolerance genetic resources and candidate genes in rice.In this study,a method was established based on image recognition technology to measure the green leaf rate of rice under PEG-6000(polyethylene glycol)simulated drought stress and H2O2 stress treatment conditions,and the correlation analysis showed that the green leaf rate measured by image recognition technology was positively correlated with the measured green leaf rate by weight,and the correlation coefficient was greater than 0.85.This method was used to measure the green leaf rate of the'Zhenshan97B'/'IRAT109'recombinant inbred population under PEG-6000(polyethylene glycol)simulated drought stress and H2O2 treatment,and QTL mapping analysis was carried out based on the genotype data of the population,and four QTLs were detected under the two stress treatment conditions.These identified QTLs will help understand the genetic basis of drought tolerance in rice,and this study will provide genetic resources for drought tolerance breeding of rice by identifying drought tolerant materials and drought tolerance QTLs.

关 键 词：水稻 绿叶率 图像识别 耐旱性 QTL定位 

分 类 号：S511[农业科学—作物学] TP391.41[自动化与计算机技术—计算机应用技术]