在拟南芥和小桐子中超量表达rJcSPL9导致花丝缩短  

Overexpression of rJcSPL9 in Arabidopsis thaliana and Jatropha curcas Causes Shorter Filaments

作　　者：陈涛 白雪 张甜甜 唐明勇 徐增富[1,4] Chen Tao;Bai Xue;Zhang Tiantian;Tang Mingyong;Xu Zengfu(CAS Key Laboratory of Tropical Plant Resources and Sustainable Use,The Innovative Academy of Seed Design,Xishuangbanna Tropical Botanical Garden,Chinese Academy of Sciences,Mengla,666303;College of Life Sciences,University of Chinese Academy of Sciences,Beijing,100049;Center of Economic Botany,Core Botanical Gardens,Chinese Academy of Sciences,Mengla,666303;State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources,College of Forestry,Guangxi University,Nanning,530004)

机构地区：[1]中国科学院西双版纳热带植物园,种子创新研究院,热带植物资源可持续利用重点实验室,勐腊666303 [2]中国科学院大学生命科学学院,北京100049 [3]中国科学院西双版纳热带植物园,核心植物园经济植物中心,勐腊666303 [4]广西大学林学院,亚热带农业生物资源保护与利用国家重点实验室,南宁530004

出　　处：《分子植物育种》2024年第23期7791-7802,共12页Molecular Plant Breeding

基　　金：国家自然科学基金青年科学基金项目(31700273);国家自然科学基金面上项目(31771605);云南省科技厅项目(2018FB060,202003AD150011);中科院西部之光B类项目(Y9XB091)共同资助。

摘　　要：SQUAMOSA promoter binding protein-like 9(SPL9)在拟南芥中被发现参与植株的成花转变。为了研究小桐子(Jatropha curcas)SPL9基因JcSPL9的功能,本研究在小桐子和拟南芥中分别超量表达对miR156不敏感的JcSPL9同义突变体rJcSPL9(miR156-resistant Jatropha curcas SPL9)。结果表明rJcSPL9转基因拟南芥果荚中的种子数量均有不同程度的减少。苯胺蓝染色结果表明转基因拟南芥植株部分雌蕊的柱头上的花粉管萌发数量有所减少;与野生型Col-0(哥伦比亚生态型)相比,转基因株系的花丝长度较短;同样,超量表达rJcSPL9的小桐子雄花的花丝也较野生型短。此外,经过外源茉莉酸(jasmonic acid,JA)处理拟南芥后,Col-0和转基因植株的花丝都变短,而spl9-4突变体及其转基因植株(35S:rJcSPL9/spl9-4)的花丝没有变短,说明spl9-4突变体对外源JA信号不敏感。结果表明拟南芥AtSPL9在花丝长度调控途径中可能位于JA信号转导途径的下游,且rJcSPL9无法互补这一功能。超量表达rJcSPL9小桐子花芽中花丝发育相关基因MYB21(MYB domain protein 21)、MYB108、MYC2(一种bHLH类转录因子)的相对表达量出现明显提高,而超量表达rJcSPL9拟南芥花芽中这些基因的表达没有发生明显变化。综上所述,小桐子JcSPL9和拟南芥AtSPL9可能通过不同途径调控花丝长度。SQUAMOSA Promoter Binding protein-like 9(SPL9)has been found to be involved in flowering transition in Arabidopsis thaliana.In this study,a miR156-resistant Jatropha curcas SPL9(rJcSPL9)gene was overexpressed in Arabidopsis thaliana and Jatropha curcas to determine the possible functions of this gene.The results showed a decrease in seed number per silique in transgenic Arabidopsis thaliana.Aniline blue staining showed a decrease in the number of pollen tubes on the stigma of transgenic Arabidopsis plants.In comparison to the Col-0(Columbia ecotype),the filaments of transgenic lines were short in length.Likewise,the filaments male flowers of Jatropha curcas overexpressing rJcSPL9 were also short in length as compared to the wild type(WT).Additionally,after treatment with exogenous jasmonic acid(JA),the filaments of both WT and the transgenic plants were shortened,whereas the filaments in spl9-4 mutant and spl9-4 mutant bearing 35S:rJcSPL9 were not,which indicated that spl9-4 mutant was insensitive to exogenous JA signal.The results suggest that AtSPL9 may be located downstream of JA signal transduction pathway in the regulation of filament length,and rJcSPL9 could not be able to complement this function.The relative expression levels of the filament development-related genes MYB21(MYB domain protein 21),and MYB108,MYC2(a basic helix-loop-helix(bHLH)DNA-binding family protein)in the flower buds of rJcSPL9 transgenic J.curcas were significantly increased,while there was no significant change in the overexpression of these genes in the flower buds of rJcSPL9 transgenic Arabidopsis.In summary,JcSPL9 and AtSPL9 may regulate filament length through different pathways.

关 键 词：小桐子 拟南芥 茉莉酸 rJcSPL9 短花丝 种子缺失 

分 类 号：Q943.2[生物学—植物学]