甜菜源性甜菜碱联合强化训练对CSID大鼠神经可塑性和神经发生的影响  

Effects of Beet Derived Betaine on Neuroplasticity and Neurogenesis in CSID Rats Combined with Intensive Training

作　　者：韩潇[1] Han Xiao(Zhengzhou University of Science and Technology,Zhengzhou,450064)

机构地区：[1]郑州科技学院,郑州450064

出　　处：《分子植物育种》2024年第23期7896-7903,共8页Molecular Plant Breeding

摘　　要：甜菜碱(betaine,BET)是一种水溶性化合物,具有抗癫痫、神经保护和改善记忆等多种药理作用。本研究旨在考察甜菜碱联合强化训练(IT)对慢性不可预知应激(CUMS)诱导的抑郁症大鼠的抗抑郁作用及其对脑源性神经营养因子(BDNF)-酪氨酸激酶受体B(TrkB)信号通路的影响,以期开发新型抗抑郁治疗策略。本研究通过建立CUMS诱导的抑郁症大鼠模型,对大鼠进行甜菜碱(15,30和60 mg/kg)和强化训练干预。行为学评价包括蔗糖偏好实验(SPT)、旷场实验(OFT)和强迫游泳试验(FST),并记录了水平活动分数(HAS)和垂直活动分数(VAS)。通过HE染色法评价海马神经元形态,采用ELISA法检测海马中白介素(IL)-1β、IL-6和肿瘤坏死因子-α(TNF-α)水平,采用RT-qPCR和Western blot检测海马中BDNF和TrkB的mRNA和蛋白水平。结果显示,与单独使用甜菜碱或强化训练相比,甜菜碱联合强化训练显著提高了SPT、HAS和VAS分数,降低了FST评分,改善了海马神经元形态,降低了海马中IL-1β、IL-6和TNF-α水平,且显著升高了海马中BDNF和TrkB的mRNA和蛋白水平(P<0.05)。这些结果表明,甜菜碱联合强化训练可能通过抑制炎症反应和激活BDNF-TrkB信号通路,增强抑郁症大鼠的神经可塑性和神经发生,从而发挥抗抑郁作用。Betaine(BET)is a water-soluble compound with various pharmacological effects,such as anti-epileptic,neuroprotective,and memory-enhancing properties.This study aims to investigate the antidepressant effects of betaine combined with intensive training(IT)on chronic unpredictable mild stress(CUMS)-induced depressed rats and its impact on the brain-derived neurotrophic factor(BDNF)-tyrosine kinase receptor B(TrkB)signaling pathway,with the goal of developing new antidepressant strategies.A CUMS-induced depression rat model was established,and the rats were subjected to interventions with betaine(15,30 and 60 mg/kg)and intensive training.Behavioral evaluations included the sucrose preference test(SPT),open field test(OFT),and forced swimming test(FST),recording the horizontal activity score(HAS)and vertical activity score(VAS).Hippocampal neuron morphology was evaluated using HE staining.Enzyme-linked immunosorbent assay(ELISA)was used to measure hippocampal levels of interleukin(IL)-1β,IL-6,and tumor necrosis factor-α(TNF-α).RT-qPCR and Western blot were employed to detect the mRNA and protein levels of BDNF and TrkB in the hippocampus.The results showed that compared with betaine or intensive training alone,the combination of betaine and intensive training significantly increased SPT,HAS,and VAS scores,reduced FST scores,improved hippocampal neuron morphology,decreased hippocampal levels of IL-1β,IL-6,and TNF-α,and significantly increased the relative mRNA and protein levels of BDNF and TrkB in the hippocampus(P<0.05).These findings suggest that betaine combined with intensive training may exert antidepressant effects by inhibiting inflammation and activating the BDNF-TrkB signaling pathway,thereby enhancing neuroplasticity and neurogenesis in depressed rats.

关 键 词：甜菜碱 强化训练 神经可塑性 神经发生 BDNF-TrkB信号通路 

分 类 号：R285.5[医药卫生—中药学]