Nanopore ultra-long sequencing and adaptive sampling spur plant complete telomere-totelomere genome assembly  

作　　者：Dongdong Lu Caijuan Liu Wenjun Ji Ruiyan Xia Shanshan Li Yanxia Liu Naixu Liu Yongqi Liu Xing Wang Deng Bosheng Li 

机构地区：[1]Shandong Key Laboratory of Precision Molecular Crop Design and Breeding,Peking University Institute of Advanced Agricultural Sciences,Shandong Laboratory of Advanced Agricultural Sciences in Weifang,Shandong 261325,China

出　　处：《Molecular Plant》2024年第11期1773-1786,共14页分子植物（英文版）

基　　金：supported by the Key R&D Program of Shandong Province,China(grant no.ZR202211070163);the National Natural Science Foundation of China(grant nos.32170574 and 32200249);the Natural Science Foundation of Shandong Province(grant nos.ZR2023QC026 and ZR2023QC106);the Young Taishan Scholars Program and Yuandu Scholars Program.

摘　　要：The pursuit of complete telomere-to-telomere(T2T)genome assembly in plants,challenged by genomic complexity,has been advanced by Oxford Nanopore Technologies(ONT),which offers ultra-long,realtime sequencing.Despite its promise,sequencing length and gap filling remain significant challenges.This study optimized DNA extraction and library preparation,achieving DNA lengths exceeding 485 kb;average N50 read lengths of 80.57 kb,reaching up to 440 kb;and maximum reads of 5.83 Mb.Importantly,we demonstrated that combining ultra-long sequencing and adaptive sampling can effectively fill gaps during assembly,evidenced by successfully filling the remaining gaps of a near-complete Arabidopsis genome assembly and resolving the sequence of an unknown telomeric region in watermelon genome.Collectively,our strategies improve the feasibility of complete T2T genomic assemblies across various plant species,enhancing genome-based research in diverse fields.

关 键 词：complete T2T genome ultra-long sequencing nanopore sequencing adaptive sampling filling gaps 

分 类 号：Q94[生物学—植物学]