Two-step emulsification for the fabrication of homogeneous PCL microspheres encapsulating geniposidic acid with antioxidant properties  

作　　者：Hongyu Wang Peng Ye Ran Song Guiru Chen Limei Zhang Huatai Zhu Yuting Ren Jiandu Lei Jing He 

机构地区：[1]Beijing Key Laboratory of Lignocellulosic Chemistry,College of Material Science and Technology,Beijing Forestry University,Beijing,100083,China

出　　处：《Particuology》2024年第11期305-313,共9页颗粒学报（英文版）

基　　金：supported by the Fundamental Research Funds for the Central Universities(grant No.PTYX202449).

摘　　要：The mortality rate of neurological disorders is increasing globally,and natural antioxidant geniposidic acid(GPA)holds great potential in the treatment of neuronal oxidative damage.Nevertheless,its inherent instability constrains its pragmatic utilization.Herein,we introduced a drug delivery system capable of protecting unstable natural active compounds from degradation.Among the various methods for preparing drug-loaded microspheres,the emulsification-solvent evaporation technique is one of the most commonly employed due to its efficiency and simplicity.Nevertheless,this method results in microspheres with heterogeneous particle sizes.To address this limitation,we developed a two-step emulsification method involving stirring and homogenization.Using the biocompatible,synthetic,biodegradable polymer polycaprolactone(PCL)as the drug delivery carrier,we prepared GPA-loaded PCL microspheres via the two-step emulsification method.The results demonstrated that the microspheres possessed uniform particle size(polydispersity index=0.12),excellent drug loading capacity(∼4.86%),sustained drug release profiles(∼68.55%in 264 h),and biocompatibility(cell viability>85%).The in vitro tests showed that the microspheres exerted antioxidant effects by scavenging reactive oxygen species(ROS)induced by oxidative stress,thereby protecting neuronal cells from oxidative damage.This work presents a promising new approach for the treatment of neuronal oxidative damage.

关 键 词：Eucommia ulmoides Oliv. POLYCAPROLACTONE Geniposidic acid Two-step emulsification Neuronal oxidative damage Antioxidant 

分 类 号：O63[理学—高分子化学]