血清中乳酸含量的同位素稀释质谱测定方法研究及应用  

Determination of lactic acid in serum by isotopic dilution mass spectrometry and its application

作　　者：王仙霞 周冬梅 邵晶[2] 刘亚辉 杨彬[1] 苏萍 杨屹[3] 武利庆 WANG Xianxia;ZHOU Dongmei;SHAO Jing;LIU Yahui;YANG Bin;SU Ping;YANG Yi;WU Liqing(National Institute of Metrology,Beijing 100029,China;National Research Institute of Sports Medicine,Beijing 100061,China;Beijing University of Chemical Technology,Beijing 100029,China)

机构地区：[1]中国计量科学研究院,北京100029 [2]国家体育总局运动医学研究所,北京100061 [3]北京化工大学,北京100029

出　　处：《中国测试》2024年第11期65-72,共8页China Measurement & Test

基　　金：市场监管总局技术保障专项(2019YJ055)。

摘　　要：该文针对当前高准确度血乳酸计量方法研究不足、血乳酸标准物质与便携式血乳酸分析仪校准技术尚属空白的现状,重点介绍了高效液相色谱-同位素稀释质谱(HPLC-IDMS)和气相色谱-同位素稀释质谱(GC-IDMS)测定血清中乳酸含量的方法,以及血清中乳酸含量标准物质研制、便携式血乳酸分析仪的校准技术及其在保障冬奥会中的应用。结果显示HPLC-IDMS和GC-IDMS测定血乳酸的结果一致性良好,两种方法的回收率分别为98.84%~101.5%和97.02%~104.4%;方法重复性RSD分别为0.30%~1.10%和0.47%~1.38%;检测限(LOD)分别为4.8×10^(-4) mmol/L和3.8×10^(-5) mmol/L;定量限(LOQ)分别为1.6×10^(-3) mmol/L和1.3×10^(-4) mmol/L。该文建立的两种血清中乳酸含量同位素稀释质谱测定方法具有良好的准确性和重复性,结果可以溯源到SI单位。该方法可用于血清中乳酸含量标准物质的研制及便携式乳酸分析仪的校准,对于建立我国血乳酸检验结果的量值溯源传递体系、保证我国血乳酸检验结果的准确可比具有积极的意义。The present work focuses on the current situation of insufficient research on high-precision blood lactate measurement methods,lack of blood lactate reference materials and calibration technique of portable blood lactate analyzers.The methods for the determination of lactic acid in serum by high performance liquid chromatography isotope dilution mass spectrometry(HPLC-IDMS)and gas chromatography-isotope dilution mass spectrometry(GC-IDMS)were presented with theirs application in developing blood lactate reference materials and calibration technique of portable blood lactate analyzers.The results of HPLC-IDMS and GC-IDMS were in good agreement.The recoveries of HPLC-IDMS and GC-IDMS were 98.84%to 101.5%,and 97.02%to 104.4%,respectively.The repeatability RSD of the two methods was 0.30%to 1.10%,and 0.47%to 1.38%.Limit of detection(LOD)is 4.8×10^(-4) mmol/L and 3.8×10^(-5) mmol/L.Limit of quantitation(LOQ)is 1.6×10^(-3) mmol/L and 1.3×10^(-4) mmol/L,respectively.The two methods presented in this paper have good accuracy and precision with SI traceability.It is expected to benefit the value traceability and transmission system and the comparability of blood lactate test in China.

关 键 词：血清中乳酸 同位素稀释质谱 高效液相色谱 气相色谱 参考方法 标准物质 

分 类 号：TB99[一般工业技术—计量学] TQ421.31[机械工程—测试计量技术及仪器]