荧光氮掺杂碳点构建鸡肉中氟喹诺酮类药物的高通量检测方法  

High-throughput detection method for fluoroquinolones in chicken based on fluorescent nitrogen-doped carbon dots

作　　者：刘梅 米琳静 张雅欣 周怡伽 唐青愉 王艳虹 陈红 廉向金 付春梅[2] LIU Mei;MI Linjing;ZHANG Yaxin;ZHOU Yijia;TANG Qingyu;WANG Yanhong;CHEN Hong;LIAN Xiangjin;FU Chunmei(SCMPA Key Laboratory for Quality Research and Control of Chemical Medicine,Chengdu institute for drug control,Chengdu 610045,China;West China School of Pharmacy,Sichuan University,Chengdu 610041,China)

机构地区：[1]四川省药品监督管理局化学药品质量研究与控制重点实验室,成都市药品检验研究院,四川成都610045 [2]四川大学华西药学院,四川成都610041

出　　处：《中国测试》2024年第11期73-81,共9页China Measurement & Test

基　　金：成都市药品检验研究院四川省药品监督管理局化学药品质量研究与控制重点实验室开放课题(2022HZKF-01)。

摘　　要：为实现动物性食品中氟喹诺酮类药物(FQ)残留量的高通量检测,合成与FQ有特异性响应的荧光N-掺杂碳点(N-CDs);以柠檬酸为碳源,L-酪氨酸为氮源,通过一步水热法制备N-CDs。利用透射电子显微镜、紫外-可见分光光度计、荧光分光光度计等仪器对N-CDs的结构、组成和光学性质进行了研究。基于FQ对N-CDs的选择性荧光增强作用,建立FQ的含量检测方法,并将其应用于鸡肉中洛美沙星的含量分析。所制备的N-CDs的平均粒径为18.25 nm,表面有羟基、羧基、酰胺基等亲水官能团,具有良好的水溶性、耐盐性、稳定性,荧光量子产率为3.63%。测定FQ的线性范围为0.781~400μg/mL,检测限在0.114~0.582μg/mL之间(S/N=3)。用于市售鸡肉中洛美沙星的检测,加标回收率为98.59%~104.3%,RSD在0.34%~5.46%之间。该实验所建立的检测方法为动物性食品中FQ的残留检测提供一种快速、灵敏的高通量检测方法。In order to achieve high-throughput detection of fluoroquinolones(FQ)residues in animal foods,fluorescent N-doped carbon dots(N-CDs)with specific response to FQ were synthesized.N-CDs were prepared by one-step hydrothermal method using citric acid as carbon source and L-tyrosine as nitrogen source.The structure,composition and optical properties of N-CDs were studied by transmission electron microscopy,ultraviolet-visible spectrophotometer,fluorescence spectrophotometer,etc.Based on the selective fluorescence enhancement effect of FQ on N-CDs,a FQ content detection method was established and applied to the content analysis of Lomefloxacin in chicken.The average particle size of the prepared N-CDs was 18.25 nm,and the surface contained hydrophilic functional groups such as hydroxyl,carboxyl and amide groups.It had good water solubility,salt tolerance and stability,and the fluorescence quantum yield was 3.63%.The linear range of FQ was 0.781-400μg/mL,and the detection limit was 0.114-0.582μg/mL(S/N=3).The recovery rate was 98.59%-104.3%and RSD was 0.34%-5.46%for the detection of lomefloxacin in commercially available chicken.The detection method established in this experiment provides a rapid and sensitive high-throughput detection method for the detection of FQ residues in animal food.

关 键 词：荧光碳点 氟喹诺酮类 含量测定 

分 类 号：TB9[一般工业技术—计量学]