白藜芦醇调控SIRT1/STAT1通路影响肝细胞癌恶性生物学过程  

Resveratrol affects the malignant biological process of hepatocellular carcinoma by regulating SIRT1/STAT1 pathway

作　　者：袁庆功[1] 张焱[1] 李军辉[1] 杨文彬[1] YUAN Qing-gong;ZHANG Yan;Li Jun-hui;YANG Wen-bin(Department of General Surgery,the Second Affiliated Hospital of Xi'an Jiaotong University,Xi'an,Shaanxi Province 710004,China)

机构地区：[1]西安交通大学第二附属医院普通外科,陕西西安710004

出　　处：《解剖学研究》2024年第6期553-558,共6页Anatomy Research

基　　金：陕西省自然科学基础研究计划项目(2023-JC-YB-637)。

摘　　要：目的探讨白藜芦醇对肝细胞癌的作用及其相关机制,以期为肝细胞癌的治疗提供新思路。方法将HepG2细胞分为对照组、低剂量白藜芦醇组(RES‐L,10μmol/L)、高剂量组白藜芦醇(RES‐H,30μmol/L)和紫杉醇组(20μmol/L),通过MTT实验、集落形成实验、Transwell实验和细胞划痕实验分析白藜芦醇对HepG2细胞的活性、侵袭和迁移能力以及凋亡率的影响;采用实时荧光定量PCR法和蛋白免疫印迹法分析白藜芦醇对SIRT1/STAT1信号通路的影响。结果与对照组比较,白藜芦醇能够抑制HepG2细胞的活性[对照组、RES‐L组、RES‐H组、紫杉醇组分别为(100.875.78)%、(81.283.25)%、(46.368.05)%、(44.687.11)%],减少HepG2细胞集落形成数量[对照组、RES‐L组、RES‐H组、紫杉醇组分别为(102.526.82)%、(88.365.15)%、(30.266.05)%、(26.385.31)%],还能够抑制HepG2细胞的侵袭和迁移能力,促进HepG2细胞凋亡[对照组、RES‐L组、RES‐H组、紫杉醇组分别为(8.32±0.72)%、(12.16±3.05)%、(26.13±1.25)%、(41.88±6.81%)%]。另外,与对照组比较,白藜芦醇处理后,SIRT1和STAT1蛋白表达水平增加。结论白藜芦醇能够抑制HepG2细胞的恶性生物学过程,并且白藜芦醇的这一作用可能与激活SIRT1/STAT1信号通路有关。Objective To explore the effect of resveratrol on hepatocellular carcinoma and its related mech-anism,in order to provide new ideas for the treatment of hepatocellular carcinoma.Methods HepG2 cells were di-vided into control group,resveratrol low-dose group(RES-L,10μmol/L),resveratrol high-dose group(RES-H,30μmol/L)and paclitaxel group(20μmol/L).MTT assay,colony formation assay,Transwell assay and cell scratch assay were used to analyze the effect of resveratrol on the activity,invasion and migration ability and apoptosis rate of HepG2 cells.Real-time PCR and Western blot were used to analyze the effect of resveratrol on SIRT1/STAT1 sig-naling pathway.Results Compared with the control group,resveratrol could inhibit the viability of HepG2 cells(100.87%±5.78%,81.28%±3.25%,46.36%±8.05%,44.68%±7.11%in control group,RES-L,RES-H and pacli-taxel group,respectively).It also reduced the colony formation of HepG2 cells(102.52%±6.82%,88.36%±5.15%,30.26%±6.05%,26.38%±5.31%in control group,RES-L,RES-H and paclitaxel group,respectively)and inhibit-ed the invasion and migration of HepG2 cells,promoted the apoptosis of HepG2 cells(8.32%±0.72%,12.16%±3.05%,26.13%±1.25%,41.88%±6.81%)in control group,RES-L,RES-H and paclitaxel group,respectively.In addition,the protein expression levels of SIRT1 and STAT1 increased after resveratrol treatment compared with the control group.Conclusion Resveratrol can inhibit the malignant biological process of HepG2 cells,and this effect of resveratrol may be related to the activation of SIRT/STAT1 signaling pathway.

关 键 词：白藜芦醇 肝细胞癌 沉默信息调节因子1 信号转导与转录激活因子1 

分 类 号：R285[医药卫生—中药学]