Cloning of BjNAC102 Promoter and Construction of Expression Vector in Brassica juncea  

芥菜型油菜BjNAC102启动子克隆及表达载体的构建

作　　者：XIAO Xin-bo YUAN Yu-hui YOU Liang HUANG Xin CHEN Ming-zhe MA Yin-hua LIU Xian-jun CHEN Hu 肖新波;袁玉辉;游亮;黄欣;陈明哲;马银花;刘显军;陈虎(湖南人文科技学院农业与生物技术学院,湘中特色农业资源开发利用与质量安全控制湖南省普通高等学校重点实验室,湖南娄底417000;资阳区农业农村局,湖南益阳413000)

机构地区：[1]College of Agriculture and Biotechnology,Hunan University of Humanities,Science and Technology,Key Laboratory of Development and Utilization and Quality and Safety Control of Characteristic Agricultural Resources in Central Hunan of College of Hunan Province,Loudi 417000,PRC [2]Bureau of Agriculture and Rural Affairs of Ziyang District,Yiyang 413000,PRC

出　　处：《Agricultural Science & Technology》2024年第3期14-20,共7页农业科技与技术（英文版）

基　　金：Supported by Natural Science Foundation of Hunan Province(2023JJ50083,2023JJ50084);Excellent Youth Project of Hunan Provincial Department of Education(22B0844);Hunan Provincial Graduate Research Innovation Project(CX20231274)。

摘　　要：Transcription factor NAC102 plays an important role in the abiotic stress responses of plants.In this study,the promoter sequence of 3000 bp located in the upstream of the BjNAC102 gene was cloned from Brassica juncea‘Sichuan Yellow Seed’by using the homologous cloning method.The expression vector of the GUS gene driven by the BjNAC102 promoter was constructed by seamless cloning technology.The results showed that the sequence of the promoter of the BjNAC102 gene contained many cis-acting elements involved in light responsiveness,gibberellinresponsive element,and auxin-responsive element.It was speculated that BjNAC102 played an important role in the abiotic stress response in Brassica juncea.The expression vector of the promoter of the BjNAC102 gene was constructed,which layed a foundation for further studies of the expression pattern of the BjNAC102 gene in Brassica juncea.NAC102转录因子在植物非生物胁迫反应中起到重要作用。该研究以芥菜型油菜‘四川黄籽’为材料,采用同源克隆方法克隆并分析了BjNAC102基因上游3000bp的启动子序列,且利用无缝克隆技术构建由BjNAC102启动子驱动GUS基因的植物表达载体。结果表明:该基因启动子序列含有许多与应答相关的顺式作用元件,如光应答、赤霉素响应元件、生长素响应元件等,推测BjNAC102在参与芥菜型油菜非生物胁迫反应中发挥着重要作用。该研究成功构建了BjNAC102基因启动子表达载体,为进一步研究芥菜型油菜BjNAC102基因的表达模式奠定了基础。

关 键 词：Brassica juncea PROMOTER BjNAC102 Plant expression vector construction 

分 类 号：S565.4[农业科学—作物学]