Extraction Condition Optimization and Identification of Phenols from Lonicerae flos  

山银花中酚类物质提取工艺优化及其成分初鉴

作　　者：GAO Wei LI Lang YU Lian-fang DING Hui-juan DING Sheng-hua 高炜;李浪;余莲芳;丁慧娟;丁胜华(湖南省农业科学院农产品加工研究所,湖南长沙410125;岳阳市农业科学研究院,农业农村部植物新品种测试岳阳分中心,湖南岳阳414000;岳阳市检验检测中心,湖南岳阳414000)

机构地区：[1]Hunan Agricultural Products Processing Institute,Hunan Academy of Agricultural Sciences,Changsha 410125,PRC [2]Yueyang Academy of Agricultural Sciences,Yueyang Branch Center of New Plant Varieties Testing of Ministry of Agriculture and Rural Affairs,Yueyang 414000,PRC [3]Yueyang Inspection and Testing Center,Yueyang 414000,PRC

出　　处：《Agricultural Science & Technology》2024年第3期21-30,共10页农业科技与技术（英文版）

基　　金：Supported by Agricultural Science and Technology Innovation Fund Project of Hunan Province(2022CX87);Huaihua Municipal Institute of Science and Technology Cooperation Project(2022N1203);Science and Technology Talent Lifting Project of Hunan Province—Training Plan for Young and Middle-aged Scholars(2023TJ-Z01)。

摘　　要：Polyphenols were obtained from the natural dried Lonicerae flos by ultrasound-assisted extraction with ethanol as the solvent.Single factor experiment and response surface methodology were employed to optimize the extraction conditions.Ultra-performance liquid chromatrography(UPLC)-tandem mass spectrometry(MS/MS)was employed to identify polyphenols based on the plant widely targeted metabolomics database in a qualitative and quantitative manner.The results showed that the optimal extraction conditions for total phenols from Lonicerae flos were ultrasound-assisted extraction with a solid-to-liquid ratio of 10∶1 g/mL and 57%ethanol at 70 W and 60°C for 11 min.The yield of total phenols extracted under the optimal conditions reached 71.08 mg/g.The phenols in Lonicerae flos were mainly chlorogenic acid isomers,and the flavonoids were mainly nobiletin,galuteolin,and homoarbutin.以自然干燥山银花为原料,采用超声波辅助乙醇溶剂提取法,通过单因素试验,结合响应面对山银花多酚的提取工艺进行优化,并用超高效液相色谱串联质谱(UPLC-MS/MS)基于植物广泛靶向数据库对其多酚物质进行定性定量初鉴。结果表明:山银花总酚提取量的最佳工艺条件为:料(g)液(m L)比10∶1,乙醇浓度57%,超声功率70 W,提取温度60℃,提取时间11 min,此条件下山银花总酚提取量为71.08 mg/g;山银花酚酸以绿原酸同分异构体为主,黄酮以川陈皮素、木犀草苷和香叶木苷为主。

关 键 词：Lonicera flos Total phenols Ultra-performance liquid chromatrography(UPLC)-tandem mass spectrometry(MS/MS) Response surface methodology PHENOLS 

分 类 号：R284.2[医药卫生—中药学]