解冻温度和时间对湖羊细管冻精解冻效果的影响  

Effects of thawing temperatures and time on thawing of frozen semen straws of Hu ram

作　　者：张柳明 江才雨 王旭洋 孙宇轩 王健[1] 孙晓梅 李拥军[1] ZHANG Liuming;JIANG Caiyu;WANG Xuyang;SUN Yuxuan;WANG Jian;SUN Xiaomei;LI Yongjun(Key Laboratory for Animal Genetics&Molecular Breeding of Jiangsu Province,College of Animal Science and Technology,Yangzhou University,Yangzhou 225009,China)

机构地区：[1]扬州大学动物科学与技术学院,江苏省动物遗传繁育与分子设计重点实验室,江苏扬州225009

出　　处：《畜牧与兽医》2024年第12期10-15,共6页Animal Husbandry & Veterinary Medicine

基　　金：江苏现代农业产业技术体系建设项目(JATS[2023]448)。

摘　　要：为提高解冻后的湖羊精液质量,本研究探讨不同解冻温度和时间对湖羊精液冷冻保存效果的影响。细管冻精分别在45、55、65、70和75℃条件下进行不同时间的解冻,通过检测精子活率、活力和运动性能探讨各温度下不同解冻时间对细管冻精解冻效果的影响;在上述试验的基础上通过检测上述指标和质膜完整性、顶体完整性以及活性氧(ROS)水平来探究各解冻温度在最适宜解冻时间下对精液冷冻保存效果的影响。结果:在45℃条件下,解冻20 s和30 s后的精子活率、活力以及各项运动速率均显著高于10 s解冻组(P<0.05);在55℃条件下,解冻8 s后的精子活力显著高于5 s解冻组(P<0.05);在65℃和70℃条件下,解冻5 s后的精子活率和活力均显著高于其他各组(P<0.05);在75℃条件下,解冻3 s后的精子各项运动速率显著高于其他各组(P<0.05);在55℃条件下解冻8 s后的精子质膜完整率和顶体完整率均显著高于37℃解冻30 s、70℃解冻5 s和75℃解冻3 s这3组(P<0.05),且ROS水平显著低于37℃解冻30 s、45℃解冻20 s、65℃解冻30 s和75℃解冻3 s这4组(P<0.05)。综上,55℃条件下解冻8 s是最适合湖羊细管精液的解冻方式,可以获得较好的精液冷冻保存效果。In order to improve the quality of post-thaw Hu ram semen,this study exploredthe effects of different thawing temperatures and time on the effectiveness of semen cryopreservation.The frozen semen straws were thawed at 45,55,65,70 and 75℃for varying durations.The effects of different thawing time and temperatures on the thawing process of frozen semen straws were examined by assessing total sperm motility,progressive motility and kinematic parameters.Based on the above experiments,the impact of different thawing temperatures on the cryopreservation of semen at the optimal thawing time was determinedby assessing the parameters mentioned above,as well as the plasma membrane integrity,acrosome integrity,reactive oxygen species(ROS)levels.The results indicated that,under the condition of 45℃,the total sperm motility,progressive motility and various kinematic parameters after thawing for 20 s and 30 s were significantly(P<0.05)higher than those in the 10 s thawing group.Under the condition of 55℃,the sperm progressive motility after thawing for 8 s was significantly(P<0.05)higher than the 5 s thawing group.Under the conditions of 65℃and 70℃,the total sperm motility and progressive motility after tha⁃wing for 5 s were significantly(P<0.05)higher than in the other groups.Under the condition of 75℃,the various kinematic parameters of sperm after thawing for 3 s were significantly(P<0.05)higher than in the other groups.After thawing for 8 s at 55℃,the sperm plasma membrane integrity and the acrosome integrity were significantly(P<0.05)higher than in the 37℃thawing for 30 s,70℃thawing for 5 s and 75℃thawing for 3 s groups;and the ROS level was significantly(P<0.05)lower than in the 37℃thawing for 30 s,45℃thawing for 20 s,65℃thawing for 30 s and 75℃thawing for 3 s groups.In conclusion,thawing for 8 s at 55℃wasthe most appropriatemethod for tha⁃wing the frozen semen of Hu ram,which would produce ideal results in semen cryopreservation.

关 键 词：湖羊 精液冷冻保存 解冻温度 解冻时间 精液质量 

分 类 号：S826[农业科学—畜牧学]