桑树光合关键基因MnPsbA、MnRbcL和MnRCA的克隆与功能分析  

Cloning and Functional analysis of Photosynthetic-key Genes of MnPsba,MnRbcL and MnRCA In Mulberry

作　　者：李勇[1] 黄锦 宋方媛 郭沚粤 邓文[1] LI Yong;HUANG Jin;SONG Fangyuan;GUO Zhiyue;DENG Wen(Cash Crops Research Institute,Hubei Academy of Agricultural Sciences,Wuhan 430064 Hubei,China)

机构地区：[1]湖北省农业科学院经济作物研究所,湖北武汉430064

出　　处：《蚕桑通报》2024年第2期36-43,48,共9页Bulletin of Sericulture

基　　金：湖北省农业科技创新中心(2021-620-000-001-009);湖北省重点研发计划项目(2022BBA0065);国家现代农业产业技术体系建设专项(CARS-18-ZJ0208)。

摘　　要：叶绿体是叶片进行光合作用制造有机物的主体器官,PsbA、RbcL和RCA在光合作用通路中起至关重要的作用。通过生信分析发现,桑树MnPsbA、MnRbcL和MnRCA的开放阅读框长度分别为1062 bp、1428 bp和1314 bp,分别编码含353、475和437个氨基酸的蛋白,其蛋白三级结构各异,折叠程度和空间转向差异大。其中,MnPsbA含高度保守的Photo_RC结构域,MnRbcL含RuBisCO_large,而MnRCA含AAA高度保守结构域;MnPsbA、MnRbcL和MnRCA在E1桑树品种中表达量最高,而在H32桑树品种中表达较低;与野生型相比,转基因拟南芥叶片Pn峰值、ΦPSⅡ、qP、以及Chla、Chlb和总Chl含量均显著高于野生型,且转基因系RuBP酶活性也呈增高表现。以上结果表明,该3个基因均可能对桑树光合有一定程度的贡献率,通过介导增加桑叶光合速率从而提高桑叶产量。Leaf photosynthesis takes places in the chloroplastorgans,among which the PsbA,RbcL and RCA genes usually play crucial roles.By biological information analysis,three mulberry genes of MnPsbA,MnRbcL and MnRCA were identified with open reading frame length of 1062 bp,1428 bp and 1314 bp,respectively,which encoded 353,475 and 437 amino acids in length.They differed on tertiary structures including different folding degree and spatial steering.The MnPsbA contained Photo_RC conserved domain,MnRbcL contained RuBisCO_large N domain while MnRCA contained AAA domain,respectively.These three genes were highly expressed in E1 mulberry variety,but lowly in H32 mulberry variety.Compared with the wild type,the peak value of Pn,ΦPSⅡ,qP,and the content of Chla,Chlb and total Chl in transgenic Arabidopsis were significantly upregulated.Simultaneously,the RuBP enzyme activity was verified with slight increase either.All above results showed that these three genes might play roles in mulberry photosynthesis,and through which promote to improve the yield of mulberry leaves.

关 键 词：桑树 光合作用 基因克隆 功能分析 

分 类 号：S888[农业科学—特种经济动物饲养]