HPLC法检测复方金银花颗粒剂型中绿原酸的含量  

HPLC Method to Detect the Content of Chlorogenic Acid in Compound Honeysuckle Granules Forms

作　　者：李修琴 Li Xiuqin(Henan Technician College of Medicine and Health,Kaifeng,Henan 475000,China)

机构地区：[1]河南医药健康技师学院,河南开封475000

出　　处：《化工与医药工程》2024年第6期49-52,共4页Chemical and Pharmaceutical Engineering

摘　　要：采用高效液相色谱法(HPLC)检测复方金银花颗粒中绿原酸的含量。采用C18反相色谱柱,以水∶乙腈溶液为流动相,等度洗脱,检测波长327 nm。以绿原酸浓度(μg/mL)为横坐标,峰面积为纵坐标,建立绿原酸标准曲线y=14 862x-254 314,相关系数r^(2)=0.999。通过峰面积计算复方金银花颗粒剂型中绿原酸的含量为60.78μg/mL,RSD值为1.72%。本方法操作简单、快速、灵敏,重现性良好,线性关系良好,可用于绿原酸的质量控制。To determine chlorogenic acid in compound honeysuckle granules by high-performance liquid chromatography(HPLC).The C18 reversed-phase column was used with water and acetonitrile solution as the mobile phase,and the detection wavelength was 327 nm.With the concentration of chlorogenic acid(μg/mL)as the horizontal coordinate and the peak area as the vertical coordinate,the standard curve y=14862x-254314 and the correlation coefficient r^(2)=0.999 were established.The content of chlorogenic acid in the formulation of compound Honeysuckle granules was 60.78μg/mL and the RSD value was 1.72%.Conclusion:The method is simple,rapid,sensitive,with good reproducibility and linear relationship,and can be used for quality control of chlorogenic acid.

关 键 词：HPLC 复方金银花颗粒 绿原酸 含量测定 

分 类 号：R284.1[医药卫生—中药学]