Multidimensional Analgesia of Acupuncture by Increasing Expression of MD2 in Central Nervous System  

作　　者：LI Wan-rong REN Lu-lu ZHAO Tian-tian DAI Dan-qing GAO Xiao-fei LIANG Hua-zheng XIONG Li-ze 

机构地区：[1]Shanghai Key Laboratory of Anesthesiology and Brain Functional Modulation,Shanghai Fourth People's Hospital,School of Medicine,Tongji University,Shanghai 200434,China [2]Clinical Research Center for Anesthesiology and Perioperative Medicine,Shanghai Fourth People's Hospital,School of Medicine,Tongji University,Shanghai 200434,China [3]Translational Research Institute of Brain and Brain-Like Intelligence,Shanghai Fourth People's Hospital,School of Medicine,Tongji University,Shanghai 200434,China [4]Department of Anesthesiology and Perioperative Medicine,Shanghai Fourth People's Hospital,School of Medicine,Tongji University,Shanghai 200434,China [5]School of Rehabilitation,Southwest Jiaotong University,Chengdu 618099,China [6]Monash Suzhou Research Institute,Suzhou,Jiangsu Province 215125,China

出　　处：《Chinese Journal of Integrative Medicine》2024年第11期1035-1044,共10页中国结合医学杂志（英文版）

基　　金：Supported by the National Natural Science Foundation of China(Nos.82130121 and 81974535);the Second Round of the Three-Year Action Plan for"Strengthening and Promoting Traditional Chinese Medicine"of Hongkou District,Shanghai(No.HKGYQYXM-2022-06)。

摘　　要：Objective:To investigate changes of myeloid differentiation factor 2(MD2)in inflammation-induced pain and acupuncture-mediated analgesia.Methods:Mice were randomly divided into three groups by a random number table method:saline group(n=16),complete Freund's adjuvant(CFA)group(n=24)and CFA+electroacupuncture(EA)group(n=26).Inflammation-induced pain was modelled by injecting CFA to the plantar surface of the hind paw of mice and EA was applied to bilateral Zusanli(ST 36)to alleviate pain.Only mice in the CFA+EA group received EA treatment(30 min/d for 2 weeks)24 h after modelling.Mice in the saline and CFA groups received sham EA.von-Frey test and Hargreaves test were used to assess the pain threshold.Brain and spinal tissues were collected for immunofluorescence staining or Western blotting to quantify changes of MD2 expression.Results:CFA successfully induced plantar pain and EA significantly alleviated pain 3 days after modelling(P<0.01).Compared with the CFA group,the number of MD2*/c-fos* neurons was significantly increased in the dorsal horn of the spinal cord 7 and 14 days after EA,especially in laminae Ⅰ-Ⅱ。(P<0.01).The proportion of double positive cells to the number of c-fos positive cells and the mean fluorescence intensity of MD2 neurons were also significantly increased in laminae Ⅰ-Ⅱ。(P<0.01).Western blotting showed that the level of MD2 was significantly decreased by EA only in the hippocampus on day 7 and 14(both P<0.01)and no significant changes were observed in the cortex,thalamus,cerebellum,or the brainstem(P>0.05).Fluorescence staining showed significant decrease in the level of MD2 in periagueductal gray(PAG)and locus coeruleus(LC)after CFA injection on day 7(P<0.01 for PAG,P<0.05 for LC)and EA significantly reversed this decrease(P<0.01 for PAG,P<0.05 for LC).Conclusion:The unique changes of MD2 suggest that EA may exert the analgesic effect through modulating neuronal activities of the superficial laminae of the spinal cord and certain regions of the brain.

关 键 词：ELECTROACUPUNCTURE myeloid differentiation factor 2 inflammation-induced pain dorsal horn periaqueductalgray locuscoeruleus 

分 类 号：R245[医药卫生—针灸推拿学]